Effect of Tomato Seed Oil on Gamma-Irradiated Male Rats
Mostafa Ahmed Farrag Mahmoud;
Abstract
Cellular exposure to ionizing radiation leads to oxidizing events that alter atomic structure through direct interaction of radiation with target macromolecules or via products of water radiolysis. To cope with the induced stress and the changes in the redox environment, organisms elicit transient responses at the molecular, cellular and tissue levels to counteract toxic effects of radiation (Azzam et al. 2012).
Tomato seed oil (TSO) was able to counteract spontaneous and H2O2-induced oxidative stress in human macrophages, limiting intracellular ROS production and controlling oxidative stress signaling (Müller et al. 2013).
Therefore, the purpose of this study was to evaluate the radioprotective potential of TSO against gamma radiation-induced damage in adult male rats. To achieve this goal, a total of sixty five male Wistar rats weighing 135±13 g were used and divided into five groups as follows: Group I (Control; n=10): Rats were fed normal diet for 8 consecutive weeks serving as normal controls.
Group II (TSO; n=10): Rats were orally treated with TSO (1 ml/kg bw) three times weekly for 8 consecutive weeks.
Summary 122
Group III (IRR; n=15): Rats were exposed to a single 6 Gy dose whole body gamma irradiation and then sacrificed 24 h later.
Group IV (TSO+ IRR; n=15): Rats were orally treated with TSO (1 ml/kg bw) three times weekly for 8 weeks then exposed to a single dose of whole body gamma radiation (6 Gy) and sacrificed 24 h later.
Group V (IRR +TSO; n=15): Rats were exposed to a single dose of whole body gamma radiation (6 Gy) then orally treated with TSO (1 ml/kg bw) three times weekly for 8 consecutive weeks.
At the end of the experiment, whole blood was withdrawn from animals for the analysis of hepatic enzymes activities (AST & ALT), renal function (urea & creatinine), lipid profile (cholesterol, TAG, HDL-C, LDL-C, and VLDL-C), VCAM-1, testosterone and SDH levels. Tissues (liver, kidneys and testes) were excised for oxidative stress biomarkers (MDA & SOD). A part of excised tissues (liver, kidneys and testes) were used for histological examination.
Tomato seed oil (TSO) was able to counteract spontaneous and H2O2-induced oxidative stress in human macrophages, limiting intracellular ROS production and controlling oxidative stress signaling (Müller et al. 2013).
Therefore, the purpose of this study was to evaluate the radioprotective potential of TSO against gamma radiation-induced damage in adult male rats. To achieve this goal, a total of sixty five male Wistar rats weighing 135±13 g were used and divided into five groups as follows: Group I (Control; n=10): Rats were fed normal diet for 8 consecutive weeks serving as normal controls.
Group II (TSO; n=10): Rats were orally treated with TSO (1 ml/kg bw) three times weekly for 8 consecutive weeks.
Summary 122
Group III (IRR; n=15): Rats were exposed to a single 6 Gy dose whole body gamma irradiation and then sacrificed 24 h later.
Group IV (TSO+ IRR; n=15): Rats were orally treated with TSO (1 ml/kg bw) three times weekly for 8 weeks then exposed to a single dose of whole body gamma radiation (6 Gy) and sacrificed 24 h later.
Group V (IRR +TSO; n=15): Rats were exposed to a single dose of whole body gamma radiation (6 Gy) then orally treated with TSO (1 ml/kg bw) three times weekly for 8 consecutive weeks.
At the end of the experiment, whole blood was withdrawn from animals for the analysis of hepatic enzymes activities (AST & ALT), renal function (urea & creatinine), lipid profile (cholesterol, TAG, HDL-C, LDL-C, and VLDL-C), VCAM-1, testosterone and SDH levels. Tissues (liver, kidneys and testes) were excised for oxidative stress biomarkers (MDA & SOD). A part of excised tissues (liver, kidneys and testes) were used for histological examination.
Other data
| Title | Effect of Tomato Seed Oil on Gamma-Irradiated Male Rats | Other Titles | تأثيس شيت بروز انطًاطى عهى ذكوز انجسذاٌ انًشععة بأشعة جايا | Authors | Mostafa Ahmed Farrag Mahmoud | Issue Date | 2016 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| G13093.pdf | 619 kB | Adobe PDF | View/Open |
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