INFLUENCE OF IN OVO INJECTION OF SELENIUM NANOPARTICLES AND SELENOMETHIONINE ON GROWTH, DEVELOPMENT OF EMBRYOS, PHYSIOLOGICAL AND IMMUNE RESPONSE OF CHICKENS

Abstract

This study aimed to investigate the effect of in ovo injection of broiler eggs with different levels and forms of selenium on growth performance, some physiological traits and blood parameters of posthatch broiler chicks. A total of 300 eggs obtained from a commercial Arbor Acres broiler breeder flock, were used in the present study. They were divided randomly into six treatment groups, 50 eggs each. The first one was kept as a control – non injected group, the second group was injected with phosphate buffer saline (PBS) , the 3rd and 4th groups were injected with organic selenium (Se-Methionine) at 50 and 100 ug/egg , while the 5th and 6th groups were injected with selenium nanoparticles (SeNPs) at 10 and 20 ug /egg, respectively . All eggs were incubated in a forced draught incubator at the recommended temperature, relative humidity and turning patterns. The in ovo injection procedure was done at the 16th day of incubation. The results showed that supplementation of different sources of Se did not significantly affect growth performance traits. Supplementation of Nano-Se increased plasma concentrations of total protein, albumin, highdensity lipoprotein glutathione peroxidase, superoxide dismutase, triiodothyronine, triiodothyronine /Tetra-iodothironin and plasma immunoglobulins concentration (IgG. IgM and IgA), and decrease glucose and ALT. However, no significant differences in globulin, A/G ratio, cholesterol, triglyceride, AST, alkaline phosphatase, total antioxidant capacity and Tetra-iodothironin levels. Nano-Se-injected groups had higher relative weights of spleen and heart than control. It can be concluded that the use of 20 ug /egg of Nano-Se caused an