"Studies on Tumor Necrosis Factor in Murine Schistosomiasis mansoni"
GEHAN LABIB HAFEZ ABU EL-ENEIN;
Abstract
Tumor necrosis factor-a is a pleiotropic cytokine that plays a crucial role in mediating the granulomatous inflammation during schistosomiasis mansani.
This study was designed to assess the potential efficacy of administration of the hu sTNFR-II:Fc construct on the immunopathological changes of host reactivity in murine schistosomiasis mansoni. The effect of the sTNFR-II:Fc construct administration was primarily evaluated in S. mansoni egg-induced pulmonary granuloma model, and was then assessed during the acute stage of the natural S. mansoni infection.
In the lung model, female BALB/c mice were intravenously injected with viable S. mansoni eggs ( 3000 eggs/mouse). Six days post-egg injection, animals were divided into 3 groups. The first group of mice received an initial dose of 100
)lg/mouse of the sTNFR-II:Fc construct intraperitoneally (i.p.), followed 2 days later by 3 doses of the construct (each 100 )lg/mouse, i.p., on alternative days). The second group of mice received an initial dose of 300 )lg/mouse ofthe sTNFR-II:Fc construct via the i.p. route, followed 2 days later by 3 doses of the construct (each 100 )lg/mouse, i.p., on alternative days). The third group served as egg-injected controls. Fourteen days post-egg injection, the lungs and spleens of treated and untreated mice were removed for histopathological and immunological studies, respectively. The experiment was carried out 3 times with at least 9 mice in each experimental group.
In the natural infection model, female BALB/c rmce were infected subcutaneously with 40 cercariae of Puerto Rican strain of S. mansoni. 5.5-wk post-infection, mice were divided into 3 groups.
This study was designed to assess the potential efficacy of administration of the hu sTNFR-II:Fc construct on the immunopathological changes of host reactivity in murine schistosomiasis mansoni. The effect of the sTNFR-II:Fc construct administration was primarily evaluated in S. mansoni egg-induced pulmonary granuloma model, and was then assessed during the acute stage of the natural S. mansoni infection.
In the lung model, female BALB/c mice were intravenously injected with viable S. mansoni eggs ( 3000 eggs/mouse). Six days post-egg injection, animals were divided into 3 groups. The first group of mice received an initial dose of 100
)lg/mouse of the sTNFR-II:Fc construct intraperitoneally (i.p.), followed 2 days later by 3 doses of the construct (each 100 )lg/mouse, i.p., on alternative days). The second group of mice received an initial dose of 300 )lg/mouse ofthe sTNFR-II:Fc construct via the i.p. route, followed 2 days later by 3 doses of the construct (each 100 )lg/mouse, i.p., on alternative days). The third group served as egg-injected controls. Fourteen days post-egg injection, the lungs and spleens of treated and untreated mice were removed for histopathological and immunological studies, respectively. The experiment was carried out 3 times with at least 9 mice in each experimental group.
In the natural infection model, female BALB/c rmce were infected subcutaneously with 40 cercariae of Puerto Rican strain of S. mansoni. 5.5-wk post-infection, mice were divided into 3 groups.
Other data
| Title | "Studies on Tumor Necrosis Factor in Murine Schistosomiasis mansoni" | Other Titles | دراسات على عامل ورم التنكرز فى فئران التجارب المصابة بالبلهارسيا مانسونى | Authors | GEHAN LABIB HAFEZ ABU EL-ENEIN | Issue Date | 2001 |
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