IN VITRO PROPAGATION OF SOME OLIVE CULTIVARS

Abstract

Two independent experiments were carried out through the period from 2014 to 2017 in order to establish protocols for the In-vitro propagation of some hard to root cultivars. The stem node explant was used in such experiments, several sterilization materials and periods, establishment media, multiplication media, rooting media as well as acclimatization media were tested. In addition, collection date of the year were tried. However, two protocols were developed for two oil cvs. namely Coratina and Kronaki as follows. Coratina In-vitro propagation protocol: sterilization of explants with ampicillin 150 mg/ L- Multiplication on MS medium enriched with BAP at 1.5 mg./L. Rooting on MS medium supplemented by 0.1 mg/ L- NAA gave 80% - Acclimatization on peatmoss: sand: vermiculite (1:1: 1) by volume. Koronaki In-vitro propagation protocol: sterilization of explants with copper sulphate 50 mg./ L. Multiplication on OM medium supplemented with kin. 1.5 mg./L. Rooting on OM medium plus IBA at 0.5 mg/ L. gave 80% - Acclimatization on peatmos: sand (1:1) by volume. In both cvs. the proper time for explants collection was the spring season.