Experimental studies on pathological and immunological aspects on avian influenza virus in SPF chickens.
FATMA AMER ABD ELREHIM SALAMA;
Abstract
As avian influenza disease considered an important disease and has effect on poultry production so this work aimed study the pathological, immunological and molecular changes in response to different vaccine regimes of avian influenza of the SPF chickens.
This study was done on total number of one hundred male SPF divided into four groups of specific pathogen free (SPF) chicks (25 birds each) were housed in separate isolators and received same food and water ad libitum. Group I: SPF chicks not vaccinated and not challenged (negative control). Group II: not vaccinated and challenged positive control SPF chicks. Group III: SPF chicks were vaccinated with inactivated AIV H5N1 Re5 vaccine alone at day 10. Group IV: SPF chicks were prime vaccinated with recombinant fowl pox vaccine (Trovac®) AIV-H5 in day old then boosted with Inactivated AIV H5N1 Re5 vaccine at day 10.
Birds of groups II, III and IV were challenged at day 31 with purified HPAI virus (A/chicken/Egypt/128s/2012(H5N1)) which is related to subclade 2.2.1. The dose per bird was 6 log10 EID50 in 100 ul allantoic fluid by intra-nasal route.
This study was done on total number of one hundred male SPF divided into four groups of specific pathogen free (SPF) chicks (25 birds each) were housed in separate isolators and received same food and water ad libitum. Group I: SPF chicks not vaccinated and not challenged (negative control). Group II: not vaccinated and challenged positive control SPF chicks. Group III: SPF chicks were vaccinated with inactivated AIV H5N1 Re5 vaccine alone at day 10. Group IV: SPF chicks were prime vaccinated with recombinant fowl pox vaccine (Trovac®) AIV-H5 in day old then boosted with Inactivated AIV H5N1 Re5 vaccine at day 10.
Birds of groups II, III and IV were challenged at day 31 with purified HPAI virus (A/chicken/Egypt/128s/2012(H5N1)) which is related to subclade 2.2.1. The dose per bird was 6 log10 EID50 in 100 ul allantoic fluid by intra-nasal route.
Other data
| Title | Experimental studies on pathological and immunological aspects on avian influenza virus in SPF chickens. | Other Titles | دراسات تجربييه على جوانب باثولوجيه ومناعيه لفيروس انفلونزا الطيور فى الدواجن الخاليه من المسببات المرضيه | Authors | FATMA AMER ABD ELREHIM SALAMA | Issue Date | 2016 |
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