Identification Of Bacterial Pathogens In Ascitic Fluid From Cirrhotic Egyptian Patients By Use Of Microbiological Culture And PCR Based Methods
Ethar Mokhtar Fathy Badran;
Abstract
SUMMARY
T
he current study was designed to compare the usefulness of the 16S rRNA-based DNA microarray with the conventional culture method in terms of detection of bacteria, identify the current causative bacteria for ascitic fluid infection and their antibiogram and identify the possible risk factors for spontaneous ascitic fluid infection in cirrhotic patients with ascites who were attending Ain Shams University hospital and Ain Shams specialized hospital over one year (from May 2015 to May 2016).
To fulfill such purpose, we studied 85 patients with chronic liver disease and ascites, either with symptoms of spontaneous bacterial peritonitis or asymptomatic. All patients were subjected to full history taking, thorough clinical examination, full laboratory investigations including (complete blood picture with differential PNL count, liver and renal profiles, CRP, viral markers: HCV Ab, HBsAg, HBcAb), abdominal ultrasonography, ascitic fluidsampling for biochemical testing, total and differential cell count, microbiological culture (conventional culture as well as the blood culture bottle) and DNA extraction and PCR
In the current study, fifty-two patients (61%
T
he current study was designed to compare the usefulness of the 16S rRNA-based DNA microarray with the conventional culture method in terms of detection of bacteria, identify the current causative bacteria for ascitic fluid infection and their antibiogram and identify the possible risk factors for spontaneous ascitic fluid infection in cirrhotic patients with ascites who were attending Ain Shams University hospital and Ain Shams specialized hospital over one year (from May 2015 to May 2016).
To fulfill such purpose, we studied 85 patients with chronic liver disease and ascites, either with symptoms of spontaneous bacterial peritonitis or asymptomatic. All patients were subjected to full history taking, thorough clinical examination, full laboratory investigations including (complete blood picture with differential PNL count, liver and renal profiles, CRP, viral markers: HCV Ab, HBsAg, HBcAb), abdominal ultrasonography, ascitic fluidsampling for biochemical testing, total and differential cell count, microbiological culture (conventional culture as well as the blood culture bottle) and DNA extraction and PCR
In the current study, fifty-two patients (61%
Other data
| Title | Identification Of Bacterial Pathogens In Ascitic Fluid From Cirrhotic Egyptian Patients By Use Of Microbiological Culture And PCR Based Methods | Other Titles | التعرف على البكتريا الممرضة في السائل البريتوني للمرضى المصريين الذين يعانون من تليف الكبد باستخدام المزارع البكتيرية وسلسله تفاعل البوليمراز | Authors | Ethar Mokhtar Fathy Badran | Issue Date | 2017 |
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