Effect of Propolis and Bee Venom against Some Pathogenic Bacteria
Basma Ibrahim Mohamed Hussein;
Abstract
Summary
he present study aimed to investigate the correlation between the major constituents of the examinedsamples
of Propolis and Bee venom of Apis mellifera carnica and their antibacterial activity on selected human pathogenic bacteria (clinical isolates from Al-Azharhospital, faculty of medicine).
Propolis and Bee venom were analyzed using GC/MS and HPLC,respectively.
Agar-well diffusion method was used to demonstrate the antibacterial activity of both propolis and bee venom on the selected strains of bacteria which were; methicillin resistant Staphylococcus aureus; MRSA (represented the Gram positive bacteria), Proteus sp., E.coli and Salmonella sp (represented Gram negativebacteria).
For Propolis analysis, GC/MS results cleared that EEP contained phenolic compounds as; some esters of aliphatic acids, some flavonoides and some new compounds were reported for the first time in Egyptian Propolis which thought to cause its antibacterial activity on the selectedbacteria.
Propolis had a noticeable effect on Gram positive bacteria (MRSA), while low to moderate effect on Gram negative bacteria (Proteus sp., E.coli, Salmonella sp.) in low concentrations.
MRSA treated with Propolis had been Investigated by transmission electron microscope to show the mode of action of Propolis, and found that inhibition of the cell division took place then non-separation of daughter cells which lead to autolysis of bacterialcells.
Qualitative High performance liquid chromatography analysis of Bee venom was done; the major components were detected by HPLC in the analyzed sample; Melittin (52.1%), Phospholipase A2(15.91%), Apamin (2.3%), also Acute toxicity of Bee Venom was represented by LD50 which was equal to290µg/mouse.
Different concentrations of bee venom (5%, 2.5%, and 1.25%) were done to study its antibacterial activity. For MRSA; the inhibition zone found was as the same as in all conc., while Gram negative bacteria (Proteus sp., E.coli, Salmonella sp.) exhibited a wider inhibition zone in the higher conc., then become smaller as the conc.decreased.
From the overall results, Propolis and bee venom could beused as alternative naturalantibiotics to help for treating human pathogenic resistance bacteria .
he present study aimed to investigate the correlation between the major constituents of the examinedsamples
of Propolis and Bee venom of Apis mellifera carnica and their antibacterial activity on selected human pathogenic bacteria (clinical isolates from Al-Azharhospital, faculty of medicine).
Propolis and Bee venom were analyzed using GC/MS and HPLC,respectively.
Agar-well diffusion method was used to demonstrate the antibacterial activity of both propolis and bee venom on the selected strains of bacteria which were; methicillin resistant Staphylococcus aureus; MRSA (represented the Gram positive bacteria), Proteus sp., E.coli and Salmonella sp (represented Gram negativebacteria).
For Propolis analysis, GC/MS results cleared that EEP contained phenolic compounds as; some esters of aliphatic acids, some flavonoides and some new compounds were reported for the first time in Egyptian Propolis which thought to cause its antibacterial activity on the selectedbacteria.
Propolis had a noticeable effect on Gram positive bacteria (MRSA), while low to moderate effect on Gram negative bacteria (Proteus sp., E.coli, Salmonella sp.) in low concentrations.
MRSA treated with Propolis had been Investigated by transmission electron microscope to show the mode of action of Propolis, and found that inhibition of the cell division took place then non-separation of daughter cells which lead to autolysis of bacterialcells.
Qualitative High performance liquid chromatography analysis of Bee venom was done; the major components were detected by HPLC in the analyzed sample; Melittin (52.1%), Phospholipase A2(15.91%), Apamin (2.3%), also Acute toxicity of Bee Venom was represented by LD50 which was equal to290µg/mouse.
Different concentrations of bee venom (5%, 2.5%, and 1.25%) were done to study its antibacterial activity. For MRSA; the inhibition zone found was as the same as in all conc., while Gram negative bacteria (Proteus sp., E.coli, Salmonella sp.) exhibited a wider inhibition zone in the higher conc., then become smaller as the conc.decreased.
From the overall results, Propolis and bee venom could beused as alternative naturalantibiotics to help for treating human pathogenic resistance bacteria .
Other data
| Title | Effect of Propolis and Bee Venom against Some Pathogenic Bacteria | Other Titles | تأثير البروبوليس وسم النحل ضد بعض البكتيريا المسببة للأمراض | Authors | Basma Ibrahim Mohamed Hussein | Issue Date | 2015 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| G11150.pdf | 958.24 kB | Adobe PDF | View/Open |
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