Phenotypic Comparison between Adherence and Magnetic Beads Isolation of Monocyte Derived Dendritic Cells

Abstract

endritic cells are professional APCs able to modulate adaptive responses through recognition of pathogens, directly or indirectly, by sensing perturbations in the microenvironment such as infectious agents, cell damage, or inflammation. DCs represent the most potent APCs in the immune system, with the unique ability to induce not only primary immune responses against invading pathogens but also immunological tolerance. Human DC can be generated in vitro from peripheral blood CD14+ monocytes, termed MoDC, or from CD34+ progenitors. Recently, it has been shown that DC can be isolated directly from peripheral blood by using BDCA but this method is not common due to low concentration of DCs in peripheral blood. CD14+ cells can be isolated either by plastic adherence or by a positive selection by using MACS method to be widely used for widespread uses in medical practice such as immunotherapy in cancer, some infectious diseases and autoimmune diseases. The aim of this study was to investigate which method of monocyte isolation, adherence method or MACS is the best as regards generation of DCs.