Diagnostic value of polymerase chain reaction of Salmonella enteritidis in poultry and surrounding People

Abstract

In the most countries of the world poultry industry contributes in solving the problem of animal food shortage as poultry meat contains essential amino acids and many vitamins and minerals which are essential for human nutrition (Mountney, 1966). Poultry have been implicated as a major source of Salmonella contaminated food products such as poultry egg or meat that cause human Salmonellosis, food borne illness (Lampel et al., 1996 and Humphrey, 1999) as all species and strains of Salmonella are possible pathogens for man

. . (Kwang et al., 1996).

... · : ..' . . , .. .:. ·..; .: ... .··.' ·..·. .;··

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Avian Salmonellosis is one of the economic problems include all phases of

· industry from production to marketing (St. louis et al., 1988; Stinert et al.,

.. : 1990; Nagaraja et al., 1991) as poultry, red meat and their products have been .. . >.:.... ··: . . conside ed the primary source of food borne Salmonellosis also poultry products .· .. < ';_·.:·are the most common vehicles of human Salmonellosis all over the world (Slavik ·. '• , .et. al.,. 19..95; Uyttendaele et al., 1998 and Bermudez et al., 2000).

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The most common serotypes isolated from humans are Salmonella

· Typhimurium and Salmonella Enteritidis but, the outbreaks of S. Enteritidis in humans increased from year to year (Perals and Audicana, 1988) where the most frequent causes of Salmonella infection reported in humans has been through .the handling of raw poultry carcasses and products in addition to the · consumption of under cooked poultry meat (Panisello et al. 2000).

.. S. Enteritidis considered an internationally important human pathogen that

causes Salmonellosis (which may lead to food poisoning) outbreaks in many countries (Humphrey et al., 1996; Flensburg, 1999 and Jorgenson et al., 2002) and only rose to its predominant position in man and poultry in the mid-

.· 1980s as a result of vertical and horizontal transmission within and between large