Effect of Adipose-derived Stem Cells on Induced Photoaging in The Skin of Adult Guinea Pig. Histological and Immunohistochemical Study.

Abstract

Repeated exposure to UV radiation causes damaging effects on the skin which is called photoaging. Current available treatments do not produce satisfactory results. Adipose-derived stem cells are available in abundant quantities, harvested by a minimally invasive procedure, could be safely and effectively transplanted and differentiated along multiple cell lineages. The use of ASCs in treatment of skin diseases has been investigated. The main objective of the present study was to evaluate the efficacy of adult ASCs in improving photodamage. Twenty adult female guinea pigs of average weight 300 grams were used after gentle shaving of the hair over their back at a surface area measuring 2 cm x 2 cm. They were divided into 2 equal groups: Group I: “Control group”: consisted of ten guinea pigs and was further subdivided into two subgroups, five animals each. • Subgroup IA: Guinea pigs were left undisturbed in their cages. • Subgroup IB: Guinea pigs were injected with 0.8ml PBS intradermaly in the shaved thin skin area. Group II: “Photoaged group”: consisted of ten guinea pigs. The thin skin of the shaved areas of these guinea pigs was exposed to UVA + UVB irradiation, five days per week for two weeks. This group was further subdivided into two subg