Detailed Histological and Histochemical Studies on the lnterphotoreceptor Tissue Components of the Retina in Certain Animals
Azza Saleh Meawad Embaby;
Abstract
The retina is very thin delicate, transparent tissue which lines the inner coat of eye. The internal aspect of the retina is in contact with the vitreous body and its external aspect is adjacent to tl1e retin;;tl P.igment epithehum separated by q potential space, the mtraretmal space, mterphotoreceptor matnx surrounds the ellipsoid portion of inner and outer segments of photoreceptor celfs IPM is a major route by which nutrients and metabolites pass between photoreceptor cells and their major vascular supply.
The aim of this work was to clanfy the nature and distribution of the interphotoreceptor tissue elements present between the different layers of the retina in certain animals.
Fourty adult, atmarent healthy animals were used in this study. They were as tollows: Group I was formed of ten albino rats, group II was formed of ten cat§_, grmw III was formed of ten rabbifs and group IV was formed or ten Guinea pigs. They were left for ten days feeding on ordinary diet, alivecf in open air and inspected daily to excfude any pafholog1cal conditions in their eyes. They were sacrificed and tfie eye oalls of each animal were d1ssected out and was fixed in Bouin's for 24 hours. Sections were then cut at 5 !-Ll11 thickness. Sections were stained by Hx and E, Alcian blue, periodic acid schiff (PAS) reaction Mallory tnchome stain and silver impregnation stains for reticular fibres.
Image analyzer computer system was used to measure the total and 12artial thickness of the retinae of the four groups of animals. The image analyzer was also used to measure the degrees of reactivity of both alcian blue and PAS reaction in the stamed slides./ .
The Hx, E stained sections of the retinae of all groups of animals showed that Muller cells in rats and cats were small and long but in rabbits and guinea pigs were short large and follow tortuous course. In rats and cafs, the matrix of the outer nuclear layer was large in thickness. But in rabbits and guinea pigs, the matrix of the outer nuclear layer was small in th1ckness.
The aim of this work was to clanfy the nature and distribution of the interphotoreceptor tissue elements present between the different layers of the retina in certain animals.
Fourty adult, atmarent healthy animals were used in this study. They were as tollows: Group I was formed of ten albino rats, group II was formed of ten cat§_, grmw III was formed of ten rabbifs and group IV was formed or ten Guinea pigs. They were left for ten days feeding on ordinary diet, alivecf in open air and inspected daily to excfude any pafholog1cal conditions in their eyes. They were sacrificed and tfie eye oalls of each animal were d1ssected out and was fixed in Bouin's for 24 hours. Sections were then cut at 5 !-Ll11 thickness. Sections were stained by Hx and E, Alcian blue, periodic acid schiff (PAS) reaction Mallory tnchome stain and silver impregnation stains for reticular fibres.
Image analyzer computer system was used to measure the total and 12artial thickness of the retinae of the four groups of animals. The image analyzer was also used to measure the degrees of reactivity of both alcian blue and PAS reaction in the stamed slides./ .
The Hx, E stained sections of the retinae of all groups of animals showed that Muller cells in rats and cats were small and long but in rabbits and guinea pigs were short large and follow tortuous course. In rats and cafs, the matrix of the outer nuclear layer was large in thickness. But in rabbits and guinea pigs, the matrix of the outer nuclear layer was small in th1ckness.
Other data
| Title | Detailed Histological and Histochemical Studies on the lnterphotoreceptor Tissue Components of the Retina in Certain Animals | Other Titles | دراسة هستولوجية وهستوكيميائية على النسج الموجودة بين الخلايا الشبكية فى بعض الحيوانات | Authors | Azza Saleh Meawad Embaby | Issue Date | 2000 |
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