is Comparative studies on carrot virus Y affecting umberlliferae plants in Egypt

Abstract

ELISA test using specific antiserum for CarVY gave positive results with CarVY-infected plants. CarVY was isolated in pure form by single local lesion technique (back inoculation test) using

Chenopodium quinoa as a local lesion host.

2- Identification

The virus isolate was identified as being CarVY according to host range, symptomatology, mode of transmission, cytopathological studies by using electron microscope and serological tests such as DAS-ELISA.

2.1- Host range and symptomatology

CarVY was transmitted mechanically to four indicator host plants Chenopodium amaranticolor, Chenopodium quinoa, Nicotiana glutinosa and Nicotiana tabacum using sap inoculation from the infected carrot leaves. Chlorotic and necrotic local lesions appeared on the inoculated leaves of Chenopodium amaranticolor and Chenopodium quinoa. Chlorotic and necrotic local lesions following by systemic symptoms were appeared on Nicotiana glutinosa and Nicotiana tabacum after mechanical inoculation with CarVY isolate.

2.2- Mode of transmission

2.2.1- Mechanical transmission CarVY was successfully transmitted by mechanical transmission through sap inoculation to the indicator host plants.

2.3- Cytopathological studies The virus particles and their affection on cytological structure of the infected plant cells were studied using electron microscopy.

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