Oxidative Stress in Segmental Versus Non Segmental Vitiligo

Abstract

Vitiligo is an acquired depigmentary disorder characterized by destruction of the epidermal melanocytes leading to the loss of the skin color. Oxidative stress has a major role in the aetiopathogenesis and in melanocytic destruction due to its accumulation in the melanocytes and the hazardous effects to all compartments of the cell. In our study we measured epidermal H2O2 in the lesional versus non-lesional skin of SV and NSV patients . Our study was conducted to 30 patients suffering from vitiligo 15 were SV and the others were NSV. They were all biopsied 2 biopsies one lesional from the margin of a lesion and the other non-lesional 15 cm from a lesion. All biopsies were refrigerated in -60 for 1-5 months then they were subjected to tissue homogenization by adding sulfur hydrogen, spin at 10000g for 5 minutes. The yellow pale supernatant was assayed by Cell Biolabs’ OxiSelectTM Hydrogen Peroxide Assay Kit USA which is a quantitative assay for measuring hydrogen peroxide for both aqueous and lipid solutions.