Susceptibility of mature E. Faecalis biofilm to anexperimental irrigating solution (An In Vitro Study)
Safaa Fouad Sayed Abo Samra;
Abstract
The aim of this study was to evaluate susceptibility of mature E.Faecalis biofilm to an experimental irrigating solution in vitro.
A total of seventy single rooted human teeth were used in this study Teeth were decoronated to a length of 16mm, cleaned and shaped, longitudinal grooves made along buccal and lingual surfaces of teeth before preparation and the teeth were divided into 7 groups, 6 experimental groups (n=10) and one control group, each group was divided into two sub-groups (sub-group A with sonic activation by vibringe and sub-group B without activation). The control group and experimental groups were incubated with previously cultured Enterococcus faecalis to form biofilm for 3 weeks at 37˚c incubator.
Six samples were scanned for evaluating the biofilm development by SEM examination at three times intervals (3,10,21 days). The 3-week-old bacterial biofilm grown on samples was observed for thickness, homogeneity, and presence of an extracellular polymeric substance using scanning electrom microscopy.
After 21 days of incubation. Each tooth received 10 ml of irrigant (2ml/30 sec.) with total irrigation time of 2.5 min. The vibringe was inserted till 1mm short of the working length and moved gently in 2-3 vertical strokes without existing the canal.
A total of seventy single rooted human teeth were used in this study Teeth were decoronated to a length of 16mm, cleaned and shaped, longitudinal grooves made along buccal and lingual surfaces of teeth before preparation and the teeth were divided into 7 groups, 6 experimental groups (n=10) and one control group, each group was divided into two sub-groups (sub-group A with sonic activation by vibringe and sub-group B without activation). The control group and experimental groups were incubated with previously cultured Enterococcus faecalis to form biofilm for 3 weeks at 37˚c incubator.
Six samples were scanned for evaluating the biofilm development by SEM examination at three times intervals (3,10,21 days). The 3-week-old bacterial biofilm grown on samples was observed for thickness, homogeneity, and presence of an extracellular polymeric substance using scanning electrom microscopy.
After 21 days of incubation. Each tooth received 10 ml of irrigant (2ml/30 sec.) with total irrigation time of 2.5 min. The vibringe was inserted till 1mm short of the working length and moved gently in 2-3 vertical strokes without existing the canal.
Other data
| Title | Susceptibility of mature E. Faecalis biofilm to anexperimental irrigating solution (An In Vitro Study) | Other Titles | مدى قابلية المكورات المعوية البرازية الناضجة الى سائل شطف تجريبى(دراسة معملية) | Authors | Safaa Fouad Sayed Abo Samra | Issue Date | 2017 |
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