DIFFERENTIATION BETWEEN REACTIVE GLIOSIS AND DIFFUSE ASTROCYTOMA USING IMMUNOHISTOCHEMISTRY WITH AN ANTIBODY SPECIFIC FOR COMMON MUTANT FORM OF IDH1
Hussein Elsayed Hussein Elsayed;
Abstract
Gliosis ( Astrocytosis ) is a process leading to scars in the central nervous system that involves the production of a dense fibrous network of neuroglia ( supporting cells ) in areas of damage.
Gliosis is a prominent feature of many diseases of the central nervous system including multiple sclerosis and stroke. After a stroke, neurons die and disappear with replacement by gliosis.
Astrocytoma ( Glioma ) is the general term applied to a diversity of glial tumors spanning from WHO GRADE I to GRADE IV , according to cellular features in each grade.
Astrocytic nuclear atypia is difficult to define and requires experience and a keen awareness of the clinical circumstances for which the tissue is being examined.
Nuclear atypia may be difficult to be recognized when compared with reactive gliosis. Astrocytic nuclear atypia is characterized by a comperatively larger nuclear size and icreased nucleo cytoplasmic ratio,angular nuclear profile while in gliosis smoother round uniform contour of the nuclei are observed.
Reactive gliosis may show mitotic activity of reactive astrocytes, a characteristic feature of demylinating disease.
The aim of this work is to evaluate the role of immunohistochemistry with an antibody specific for the common mutant form of IDH1 is of great value in differentiation between reactive gliosis and diffuse astrocytoma.
The material consists of formalin fixed paraffin embedded archival material selected from the pathology files in Ain Shams University Hospitals consisted of 50 tissue biopsies ( 25 biopsies of reactive gliosis and 25 biopsies of diffuse astrocytoma, WHO grade II ).
The biopsies are immunohistochemically stained with an antibody specific for common mutant form of IDH 1.
Ki67 is also used for the biopsies as a proliferation marker.
The results are correlated with MRI finding of the samples.
The clinical data were obtained from the pathology sheets of the patients. These data included age of the patients, sex and the exact site of the tumor.
Isocitrate dehydrogenase enzymes catalyze the oxidative decarboxylation of isocitrate to produce alfa ketoglutarate.
The identification of mutations in a specific isoform of IDH1 gene in astrocytoma suggest that this enzyme may be promoting oncogenesis
Our study showed that IDH1 mutations commonly occur in diffuse astrocytoma tumors suggesting the possible role of IDH1 in the pathogenicity and malignant progression of diffuse astrocytomas.
All samples (n = 50) examined for mutations of the IDH1 gene were analyzed for IDH1 mutant protein expression.
As regards reactive gliosis ,all cases(25) are negative for IDH1 stain.
In contrast, 24 cases of diffuse astrocytoma (96%) are positive for IDH1stain and only one case ( 4%) is negative .
Gliosis is a prominent feature of many diseases of the central nervous system including multiple sclerosis and stroke. After a stroke, neurons die and disappear with replacement by gliosis.
Astrocytoma ( Glioma ) is the general term applied to a diversity of glial tumors spanning from WHO GRADE I to GRADE IV , according to cellular features in each grade.
Astrocytic nuclear atypia is difficult to define and requires experience and a keen awareness of the clinical circumstances for which the tissue is being examined.
Nuclear atypia may be difficult to be recognized when compared with reactive gliosis. Astrocytic nuclear atypia is characterized by a comperatively larger nuclear size and icreased nucleo cytoplasmic ratio,angular nuclear profile while in gliosis smoother round uniform contour of the nuclei are observed.
Reactive gliosis may show mitotic activity of reactive astrocytes, a characteristic feature of demylinating disease.
The aim of this work is to evaluate the role of immunohistochemistry with an antibody specific for the common mutant form of IDH1 is of great value in differentiation between reactive gliosis and diffuse astrocytoma.
The material consists of formalin fixed paraffin embedded archival material selected from the pathology files in Ain Shams University Hospitals consisted of 50 tissue biopsies ( 25 biopsies of reactive gliosis and 25 biopsies of diffuse astrocytoma, WHO grade II ).
The biopsies are immunohistochemically stained with an antibody specific for common mutant form of IDH 1.
Ki67 is also used for the biopsies as a proliferation marker.
The results are correlated with MRI finding of the samples.
The clinical data were obtained from the pathology sheets of the patients. These data included age of the patients, sex and the exact site of the tumor.
Isocitrate dehydrogenase enzymes catalyze the oxidative decarboxylation of isocitrate to produce alfa ketoglutarate.
The identification of mutations in a specific isoform of IDH1 gene in astrocytoma suggest that this enzyme may be promoting oncogenesis
Our study showed that IDH1 mutations commonly occur in diffuse astrocytoma tumors suggesting the possible role of IDH1 in the pathogenicity and malignant progression of diffuse astrocytomas.
All samples (n = 50) examined for mutations of the IDH1 gene were analyzed for IDH1 mutant protein expression.
As regards reactive gliosis ,all cases(25) are negative for IDH1 stain.
In contrast, 24 cases of diffuse astrocytoma (96%) are positive for IDH1stain and only one case ( 4%) is negative .
Other data
| Title | DIFFERENTIATION BETWEEN REACTIVE GLIOSIS AND DIFFUSE ASTROCYTOMA USING IMMUNOHISTOCHEMISTRY WITH AN ANTIBODY SPECIFIC FOR COMMON MUTANT FORM OF IDH1 | Other Titles | دراسه باثولوجيه للتمييز بين مرض الدباق المتفاعل و مرض الورم النجمى المنتشر باستخدام الصبغه المناعيه(IDH1) المتحول | Authors | Hussein Elsayed Hussein Elsayed | Issue Date | 2016 |
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