Study of Human Cord Blood Stem Cell Separation, Transdifferantiation into Neurocytes and Verification of the Transformed Cells in vitro

Abstract

Umbilical cord blood provides a rich source of stem cells with several advantages. Basically, these cells serve as a sort of repair system for the body, and since cell loss is a common factor in many types of nervous system disorders, therefore it is important to assess the availability of UCB derived stem cells for transplantation to generate new neurons or glial cells, which could be a promising therapeutic approach for future treatment of traumatic brain injury and neurodegenerative diseases. The aim of the present study was to assess the ability of human cord blood stem cell to transdifferentiate into neurocytes and verification of the transformed cells in vitro which may be needed for transplantation in the future. The study was conducted on cord blood samples from 20 newborn babies collected from obstetric ward in Ain Shams Maternity Hospital. MSCs separation was done from freshly isolated MNC suspensions in a primary culture for 2weeks. MSCs were identified before induction by flow-cytometric analysis of CD29 and cytochemical stain for PAS. MSCs were then induced to transdifferentiate into neurocyte by neurogenic