EVALUATION OF THE CHICKEN IMMUNE RE- SPONSE AGAINST THE DIFFERENT TYPES OF RECOMBINANT AVIAN INFLUENZA VACCINES IN COMPARISON TO THE ORDINARY INACTIVATED ONES
DALIA MOHAMED OMAR GAAFAR;
Abstract
Highly Pathogenic Avian Influenza (HPAI) H5N1 virus, poses a serious threat to animal and public health worldwide. Since late 2003, the virus has spread to over 60 countries in Asia, Europe and Africa leading to death or culling of hundreds of millions of poultry and occasional transmission to humans revealing its pandemic potential. Effective vaccination against HPAI H5N1 would protect commercial poultry and would thus provide an important control measure by reducing the likelihood of bird-to-bird as well as bird-to-human transmission. Therefore, the development of efficacious influenza vaccines is of high veterinary and public health importance.
So, the present study was designed for detection of the effectiveness of different recombinant AI vaccine types in prevention of poultry infection with AI and reducing the environmental contamination through:
1. Studying the immune response of the recombinant AI vaccines in protection of chicken against avian flu.
2. Comparative study between the immune response against the recombinant and inactivated AI vaccines.
3. Studying the efficacy of the recombinant AI vaccines against classical and variant clades of highly pathogenic AI challenge virus.
4. Studying the efficacy of recombinant AI vaccines for reduction of viral shedding.
5. Isolation of new local AIV isolate in Egypt to know antigenic variation between the new isolate 2016 and other isolates in 2008, 2010, 2012 and 2015.
From this study, the following points were performed:
A) Isolation and identification of newly avian influenza (AI) strain from infected Egyptian flocks farms:
1. Fresh samples of several dead and sick birds from infected flock (had a history of AI clinical signs and gross lesion) were collected. The isolated AI viruses cause embryo deaths as early as 24 - 48 hours post inoculation and all samples showing complete positive haemagglutination.
2. The isolated virus was serologically belonged to H5-AIV positive while they were ND and EDS negative. The result of HI test for the AIV new Egyptian isolate was 10.3 log2 H5N1 antisera.
3. The HA titer for AI virus was 7 log2.
4. The intravenous pathogenicity index (IVPI) value of the AI virus was 2.1. So, it considered to be a highly pathogenic AIV for chicken.
5. The AIV titer was expressed as EID50 and ranged from 1011.5 for AI virus.
6. The results of RT-PCR test for M gene confirmed that the isolate
So, the present study was designed for detection of the effectiveness of different recombinant AI vaccine types in prevention of poultry infection with AI and reducing the environmental contamination through:
1. Studying the immune response of the recombinant AI vaccines in protection of chicken against avian flu.
2. Comparative study between the immune response against the recombinant and inactivated AI vaccines.
3. Studying the efficacy of the recombinant AI vaccines against classical and variant clades of highly pathogenic AI challenge virus.
4. Studying the efficacy of recombinant AI vaccines for reduction of viral shedding.
5. Isolation of new local AIV isolate in Egypt to know antigenic variation between the new isolate 2016 and other isolates in 2008, 2010, 2012 and 2015.
From this study, the following points were performed:
A) Isolation and identification of newly avian influenza (AI) strain from infected Egyptian flocks farms:
1. Fresh samples of several dead and sick birds from infected flock (had a history of AI clinical signs and gross lesion) were collected. The isolated AI viruses cause embryo deaths as early as 24 - 48 hours post inoculation and all samples showing complete positive haemagglutination.
2. The isolated virus was serologically belonged to H5-AIV positive while they were ND and EDS negative. The result of HI test for the AIV new Egyptian isolate was 10.3 log2 H5N1 antisera.
3. The HA titer for AI virus was 7 log2.
4. The intravenous pathogenicity index (IVPI) value of the AI virus was 2.1. So, it considered to be a highly pathogenic AIV for chicken.
5. The AIV titer was expressed as EID50 and ranged from 1011.5 for AI virus.
6. The results of RT-PCR test for M gene confirmed that the isolate
Other data
| Title | EVALUATION OF THE CHICKEN IMMUNE RE- SPONSE AGAINST THE DIFFERENT TYPES OF RECOMBINANT AVIAN INFLUENZA VACCINES IN COMPARISON TO THE ORDINARY INACTIVATED ONES | Authors | DALIA MOHAMED OMAR GAAFAR | Issue Date | 2017 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.