Effect of Moringa Oleifera Leaves Extract on Submandibular and Sublingual Salivary Glands of Streptozotocin Induced Diabetic Albino Rats
Safaa Ismail Hussein Wahbi;
Abstract
Streptozotocin destroys pancreatic beta cells which are sensitive to damage by free radicals. It has been suggested that the antihyperglycemic effect of Moringa Oleifera is due to terpenoids that stimulate beta cells resulting in secretion of insulin. So the aim of this study was to evaluate the possible antidiabetic effect of Moringa Oleifera leaves extract on the submandibular and sublingual salivary glands of Streptozotocin-induced diabetic Albino rats.
Materials and methods: Forty two male Albino rats weighing between 200-250 grams were divided into 3 groups, control, diabetic and Moringa Oleifera treated. In each group, rats were divided into 2 subgroups, A and B. Rats in control group were injected with 1 ml/kg of citrate buffer. In diabetic and Moringa treated groups, rats were injected with STZ (40 mg/kg). Once diagnosed diabetic, rats of Moringa treated subgroups received an oral daily dose of Moringa Oleifera extract (250 mg/kg). In subgroups A: rats were kept for 21 days. While, in subgroups B: rats were kept for 28 days. The submandibular and sublingual glands were processed to be stained with Hematoxylin and Eosin and anti-active caspase-3 antibody.
Results: Examination of submandibular glands of diabetic subgroups showed cytoplasmic vacuolations in acini, intercalated and striated ducts. Convoluted tubules showed decreased cytoplasmic acidophilia and excretory ducts revealed stagnated secretion and desquamated cells. Immunohistochemical examination revealed too many diffuse positive cytoplasmic and nuclear areas in acini and striated duct, but many diffuse areas in tubules and excretory duct. In Moringa subgroups, glands showed normal architecture, except for some irregular nuclei at three weeks. By immunohistochemistry, few localized positive cytoplasmic and nuclear areas in acini, but many diffuse reactions in tubules, striated and excretory ducts were seen. Sublingual glands in diabetic subgroups showed normal appearance of acini. Intercalated ducts showed decreased cells height. Degeneration of striated ducts cells and loss of pseudo stratification in excretory ducts. Immunohistochemical examination revealed many localized positive cytoplasmic and nuclear areas in acini and demilunes but many diffuse areas in striated and excretory ducts. In Moringa subgroups, glands showed normal architecture, except for few stagnant secretions in excretory ducts. Immunohistochemical examination showed few localized positive cytoplasmic and nuclear reactions in acini and demilunes, but few diffuse areas in striated and excretory ducts.
Conclusions: Treatment of Diabetes Mellitus with Moringa Oleifera aqueous leaves extract was able to counteract the diabetic condition by decreasing fasting blood glucose level, decreasing signs of cellular degeneration and apoptosis.
Materials and methods: Forty two male Albino rats weighing between 200-250 grams were divided into 3 groups, control, diabetic and Moringa Oleifera treated. In each group, rats were divided into 2 subgroups, A and B. Rats in control group were injected with 1 ml/kg of citrate buffer. In diabetic and Moringa treated groups, rats were injected with STZ (40 mg/kg). Once diagnosed diabetic, rats of Moringa treated subgroups received an oral daily dose of Moringa Oleifera extract (250 mg/kg). In subgroups A: rats were kept for 21 days. While, in subgroups B: rats were kept for 28 days. The submandibular and sublingual glands were processed to be stained with Hematoxylin and Eosin and anti-active caspase-3 antibody.
Results: Examination of submandibular glands of diabetic subgroups showed cytoplasmic vacuolations in acini, intercalated and striated ducts. Convoluted tubules showed decreased cytoplasmic acidophilia and excretory ducts revealed stagnated secretion and desquamated cells. Immunohistochemical examination revealed too many diffuse positive cytoplasmic and nuclear areas in acini and striated duct, but many diffuse areas in tubules and excretory duct. In Moringa subgroups, glands showed normal architecture, except for some irregular nuclei at three weeks. By immunohistochemistry, few localized positive cytoplasmic and nuclear areas in acini, but many diffuse reactions in tubules, striated and excretory ducts were seen. Sublingual glands in diabetic subgroups showed normal appearance of acini. Intercalated ducts showed decreased cells height. Degeneration of striated ducts cells and loss of pseudo stratification in excretory ducts. Immunohistochemical examination revealed many localized positive cytoplasmic and nuclear areas in acini and demilunes but many diffuse areas in striated and excretory ducts. In Moringa subgroups, glands showed normal architecture, except for few stagnant secretions in excretory ducts. Immunohistochemical examination showed few localized positive cytoplasmic and nuclear reactions in acini and demilunes, but few diffuse areas in striated and excretory ducts.
Conclusions: Treatment of Diabetes Mellitus with Moringa Oleifera aqueous leaves extract was able to counteract the diabetic condition by decreasing fasting blood glucose level, decreasing signs of cellular degeneration and apoptosis.
Other data
| Title | Effect of Moringa Oleifera Leaves Extract on Submandibular and Sublingual Salivary Glands of Streptozotocin Induced Diabetic Albino Rats | Other Titles | تأثير مستخلص أوراق المورينغا أوليفيرا على الغدد تحت الفكية و تحت اللسانية في الفئران البيضاء المصابة بداء السكري المستحث بعقار الستربتوزوتوسين | Authors | Safaa Ismail Hussein Wahbi | Issue Date | 2017 |
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