A Study on The Effect of Sildenafil Citrate on Acute Renal Ischemic-Reperfusion Injury in male Rats
Suzanne Albert Naguib Estaphan;
Abstract
There is current clinical evidence that acute ischemia reperfusion of the kidney results in deterioration of its performance resulting in bad outcome of kidney transplantation operations and major operations in which the kidneys are exposed to acute ischemia reperfusion.
The present study was designed to investigate if sildenafil citrate has a protective role in renal I/R injury and to study its possible mechanisms of action with the aim of establishing a mechanism that may aid in treatment.
Our study was conducted on 60 male albino rats of average weight between 150 -200 gram and divided into the following groups:-
Group I: Control (sham- operated).
Group II: Ischemia of the kidneys for 50 minutes
Group III: I/R of the kidneys (ischemia for 50 minutes then reperfusion for 2 hours).
Group IV: SC(1mg/kg orally) +sham operated group.
Group V: Ischemia of the kidneys for 50 minutes , one hour after SC oral treatment .
Group VI: I/R of the kidneys, one hour after SC oral treatment .
At the end of experiment, rat tail systolic blood pressure was measured, blood samples were collected to measure serum creatinine and blood urea nitrogen then, the animals were sacrificed and kidneys were excised to measure NO level in renal tissues.
The study documented that I/R resulted in significant increase in the serum creatinine, blood urea nitrogen levels, rat tail systolic B.P., but resulted in significant decrease in NO level in renal tissues..
SC prophylactic treatment resulted in partial reversal of measured parameters compared to the groups untreated with the same drug. For instance, there was significant decrease in serum creatinine, blood urea nitrgen. Furthermore, there was significant increase in NO level in renal tissues in I/R after SC prophylactic treatment compared to the groups untreated with the same drug.
There was no significant difference in rat tail systolic blood pressure after SC prophylactic treatment compared to the groups untreated with the same drug.
The present study was designed to investigate if sildenafil citrate has a protective role in renal I/R injury and to study its possible mechanisms of action with the aim of establishing a mechanism that may aid in treatment.
Our study was conducted on 60 male albino rats of average weight between 150 -200 gram and divided into the following groups:-
Group I: Control (sham- operated).
Group II: Ischemia of the kidneys for 50 minutes
Group III: I/R of the kidneys (ischemia for 50 minutes then reperfusion for 2 hours).
Group IV: SC(1mg/kg orally) +sham operated group.
Group V: Ischemia of the kidneys for 50 minutes , one hour after SC oral treatment .
Group VI: I/R of the kidneys, one hour after SC oral treatment .
At the end of experiment, rat tail systolic blood pressure was measured, blood samples were collected to measure serum creatinine and blood urea nitrogen then, the animals were sacrificed and kidneys were excised to measure NO level in renal tissues.
The study documented that I/R resulted in significant increase in the serum creatinine, blood urea nitrogen levels, rat tail systolic B.P., but resulted in significant decrease in NO level in renal tissues..
SC prophylactic treatment resulted in partial reversal of measured parameters compared to the groups untreated with the same drug. For instance, there was significant decrease in serum creatinine, blood urea nitrgen. Furthermore, there was significant increase in NO level in renal tissues in I/R after SC prophylactic treatment compared to the groups untreated with the same drug.
There was no significant difference in rat tail systolic blood pressure after SC prophylactic treatment compared to the groups untreated with the same drug.
Other data
| Title | A Study on The Effect of Sildenafil Citrate on Acute Renal Ischemic-Reperfusion Injury in male Rats | Other Titles | علاقة السيلدينافيل باصابة الكلى الحادة نتيجة القصور الشريانى واعادة الأرتواء فى الفئران الذكور | Authors | Suzanne Albert Naguib Estaphan | Issue Date | 2011 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| B11028.pdf | 566.27 kB | Adobe PDF | View/Open |
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