Influence of different levels of exogenous enzymes preparation at two application methods on in vitro ruminal fermentation of some fibrous feeds in sheep
H.Gado; A. Z. M. Salem; N. E. Odongo; R. Rojo; M. M. Y. Elghandour; A. Olmido;
Abstract
The present experiment was aimed at investigating the effect of different
levels of exogenous enzymes preparation (ENZ - mixture of cellulase,
xylanase, α-amylase and proteases enzymes) on in vitro gas production,
some ruminal fermentation parameters (pH, NH3-N and VFA (total and
individual acids) and fiber degradability (ADF and NDF) of three fibrous
feeds (FF - corn stover, oat straw, sugarcane bagasse) at two methods of
application (direct addition or pretreatment for 24h with ENZ) in sheep.
The levels of ENZ used were 0, 0.06, 0.12 and 0.24 g/g DM of each FF,
and ENZ was dissolved in distilled water to obtain the concentration
of each level in 1 mL. Rumen liquor was collected before the morning
eating from 4 sheep (40 ± 3.2 kg BW, fed on a total mixed ration of
50% commercial concentrate and 50% alfalfa hay) fitted with permanent
rumen cannula. In vitro gas production was recorded at 2, 4, 6, 8, 10, 12,
24, 48 and 72 h of incubation. After 72 h, the incubation was stopped
and the inoculant’s pH was determined and filtered to determine ADF
and NDF degradability, VFA (total and individual acids) and NH3-N
concentrations. In vitro organic matter digestibility and metabolizable
energy, were estimated. Data were analyzed as 4 (four doses of ENZ)
× 3 (three FF) × 2 (two methods of application) factorial experimental
design. Addition of ENZ increased (P<0.05) gas production, NH3-N and
VFA concentrations with the FF used. Acetic acid concentration was
increased with the addition or pretreatment of FF with ENZ without
effect on butyric or propionic acids concentrations. Pretreatment of FF
for 24 h with ENZ was increased the ruminal fermentation and fiber
degradability (ADF and NDF) of FF. Highest in vitro gas production and
ruminal fermentation activities were found in sugarcane bagasse compared with oat straw and corn stover. It is concluded that pretreatment
the FF with ENZ for 24 h before the in vitro incubation could improve
the ruminal fermentation and fiber degradability in sheep compared to
the direct addition in diets. Sugarcane bagasse treated with ENZ could
be considered a good roughage for ruminants.
levels of exogenous enzymes preparation (ENZ - mixture of cellulase,
xylanase, α-amylase and proteases enzymes) on in vitro gas production,
some ruminal fermentation parameters (pH, NH3-N and VFA (total and
individual acids) and fiber degradability (ADF and NDF) of three fibrous
feeds (FF - corn stover, oat straw, sugarcane bagasse) at two methods of
application (direct addition or pretreatment for 24h with ENZ) in sheep.
The levels of ENZ used were 0, 0.06, 0.12 and 0.24 g/g DM of each FF,
and ENZ was dissolved in distilled water to obtain the concentration
of each level in 1 mL. Rumen liquor was collected before the morning
eating from 4 sheep (40 ± 3.2 kg BW, fed on a total mixed ration of
50% commercial concentrate and 50% alfalfa hay) fitted with permanent
rumen cannula. In vitro gas production was recorded at 2, 4, 6, 8, 10, 12,
24, 48 and 72 h of incubation. After 72 h, the incubation was stopped
and the inoculant’s pH was determined and filtered to determine ADF
and NDF degradability, VFA (total and individual acids) and NH3-N
concentrations. In vitro organic matter digestibility and metabolizable
energy, were estimated. Data were analyzed as 4 (four doses of ENZ)
× 3 (three FF) × 2 (two methods of application) factorial experimental
design. Addition of ENZ increased (P<0.05) gas production, NH3-N and
VFA concentrations with the FF used. Acetic acid concentration was
increased with the addition or pretreatment of FF with ENZ without
effect on butyric or propionic acids concentrations. Pretreatment of FF
for 24 h with ENZ was increased the ruminal fermentation and fiber
degradability (ADF and NDF) of FF. Highest in vitro gas production and
ruminal fermentation activities were found in sugarcane bagasse compared with oat straw and corn stover. It is concluded that pretreatment
the FF with ENZ for 24 h before the in vitro incubation could improve
the ruminal fermentation and fiber degradability in sheep compared to
the direct addition in diets. Sugarcane bagasse treated with ENZ could
be considered a good roughage for ruminants.
Other data
| Title | Influence of different levels of exogenous enzymes preparation at two application methods on in vitro ruminal fermentation of some fibrous feeds in sheep | Authors | H.Gado ; A. Z. M. Salem ; N. E. Odongo ; R. Rojo ; M. M. Y. Elghandour ; A. Olmido | Keywords | exogenous enzymes;fibrous feeds;Gas production | Issue Date | 2012 | Publisher | Annual meeting of ASAS & ADSA 2012 | Journal | Annual meeting of ASAS & ADSA 2012 |
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