The effect of different doses of exogenous enzymes preparation on in vitro gas production and ruminal fermentation activities of some fibrous feeds in cows
Hany M. Gado; M. M. Y. Elghandour; C.G. Peñuelas-Rivas; M. Ronquillo; A. Z. M. Salem; N. E. Odongo;
Abstract
This study was conducted to investigate effects of different doses of
exogenous enzyme preparation (ENZ- 0, 0.06, 0.12 and 0.24 g/g DM)
on in vitro gas production and some ruminal fermentation patterns such
as truly degraded substrate (TDS), short chain fatty acids (SCFA), and
microbial protein production (MP) of 4 fibrous feeds (FF - Sugarcane
leaves, taiwan grass, sorghum straw and llanero grass) in cows. Rumen
liquor was collected before the morning feeding from 2 cows (450 ± 6
kg BW) fitted with permanent rumen cannula. In vitro gas production
(GP) was recorded at 2, 4, 6, 8, 10, 12, 24, 48 and 72 h of incubation.
After 72 h, the incubation was stopped and the inoculants pH was determined and filtered to determine TDS. Ruminal fermentation parameters
such as 24 h partitioning factor (PF24), gas yield (GY24), in vitro
organic matter digestibility (IVOMD), metabolizable energy (ME),
SCFA, and MP were also estimated. Data were analyzed as 4 (4 doses
of ENZ) x 4 (4 FF) factorial experimental design. Interaction between
FF × ENZ (P < 0.05) only occurred for gas production at 12 h (GP12)
and 24 h (GP24) of incubation, but there were no interactions after 24
h of incubation. Relative to control, addition of ENZ increased (P <
0.05) accumulated gas volume of all FF during the 72 h of incubation
compared with control (0 g ENZ/g DM). Among the FF species, gas
production was higher (P < 0.001) in sorghum straw and lower (P <
0.001) in sugarcane leaves. There was no significant effect of ENZ on
gas production parameters (i.e., b; asymptotic gas production, c; rate of
gas production and L; discrete lag time before gas production) of all FF.
Addition of ENZ doses increased (P < 0.05) gradually gas production
and this increase was higher (P < 0.05) for 0.24 g ENZ/g DM. addition
of ENZ was increased (P < 0.05) some fermentation parameters (i.e.,
IVOMD, ME, GY24 and SCFA) were higher (P < 0.05) of all FF, while
the highest (P < 0.05) increased were in sorghum straw with a lower
values in sugarcane leaves. Results indicated that exogenous enzymes
preparation probably improve the ruminal fermentation activities of
fibrous feeds in cows. The authors wish to acknowledge the financial
support from IAEA/FAO project MEX 16307.
exogenous enzyme preparation (ENZ- 0, 0.06, 0.12 and 0.24 g/g DM)
on in vitro gas production and some ruminal fermentation patterns such
as truly degraded substrate (TDS), short chain fatty acids (SCFA), and
microbial protein production (MP) of 4 fibrous feeds (FF - Sugarcane
leaves, taiwan grass, sorghum straw and llanero grass) in cows. Rumen
liquor was collected before the morning feeding from 2 cows (450 ± 6
kg BW) fitted with permanent rumen cannula. In vitro gas production
(GP) was recorded at 2, 4, 6, 8, 10, 12, 24, 48 and 72 h of incubation.
After 72 h, the incubation was stopped and the inoculants pH was determined and filtered to determine TDS. Ruminal fermentation parameters
such as 24 h partitioning factor (PF24), gas yield (GY24), in vitro
organic matter digestibility (IVOMD), metabolizable energy (ME),
SCFA, and MP were also estimated. Data were analyzed as 4 (4 doses
of ENZ) x 4 (4 FF) factorial experimental design. Interaction between
FF × ENZ (P < 0.05) only occurred for gas production at 12 h (GP12)
and 24 h (GP24) of incubation, but there were no interactions after 24
h of incubation. Relative to control, addition of ENZ increased (P <
0.05) accumulated gas volume of all FF during the 72 h of incubation
compared with control (0 g ENZ/g DM). Among the FF species, gas
production was higher (P < 0.001) in sorghum straw and lower (P <
0.001) in sugarcane leaves. There was no significant effect of ENZ on
gas production parameters (i.e., b; asymptotic gas production, c; rate of
gas production and L; discrete lag time before gas production) of all FF.
Addition of ENZ doses increased (P < 0.05) gradually gas production
and this increase was higher (P < 0.05) for 0.24 g ENZ/g DM. addition
of ENZ was increased (P < 0.05) some fermentation parameters (i.e.,
IVOMD, ME, GY24 and SCFA) were higher (P < 0.05) of all FF, while
the highest (P < 0.05) increased were in sorghum straw with a lower
values in sugarcane leaves. Results indicated that exogenous enzymes
preparation probably improve the ruminal fermentation activities of
fibrous feeds in cows. The authors wish to acknowledge the financial
support from IAEA/FAO project MEX 16307.
Other data
| Title | The effect of different doses of exogenous enzymes preparation on in vitro gas production and ruminal fermentation activities of some fibrous feeds in cows | Authors | Hany M. Gado ; M. M. Y. Elghandour ; C.G. Peñuelas-Rivas ; M. Ronquillo ; A. Z. M. Salem ; N. E. Odongo | Keywords | Exogenous enzymes;fibrous feeds;Gas production | Issue Date | 2012 | Publisher | Annual meeting of ASAS & ADSA 2012 | Journal | Annual meeting of ASAS & ADSA 2012 |
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