Effect incremental levels of exogenous enzyme preparation on extent of ruminal fermentation, nutrient digestibilties and average daily gain in steers
H.M. Gado; A. Z. M. Salem; B. E. Borhami;
Abstract
The aim of this experiment was to investigate the effect of the gradual
increasing levels of an exogenous enzymes preparation (mixture of
cellulase, xylanase, α-amylase and proteases enzymes) in the diet on
ruminal fermentations activates, nutrients digestibility and animal
performance in steers. Forty 8 steers (Baladi × Friesian, 151 ± 0.95 kg
BW) were randomly allocated in 3 experimental groups of the following
treatments (12 steers for each): 0 (control, ENZ0), 40 (ENZ40) and 60 (ENZ60) g of ENZ/head/d. A complete randomize block design was
set up and a repeated measurement in time model used to analyze the
data. The ENZ doses were mixed daily with a part of their daily ration.
Steers were fed a total mixed ration (TMR) contained: 11% cracked
corn, 8.5% agwa (minced date), 26.5% biscuits (bakery waste), 10%
sugar cane molasses, 19% sesame cake, 2% soybean meal, 6% beans,
14.2% rihan straw, 1% salt, 1% limestone and, 0.3 mixture of mineral
and vitamins. Steers were fed the TMR for 206 d as experimental
period. Feed and orts samples were collected twice weekly to calculate
dry matter intake and animals were weighted each 2 weeks during the
experiment. At the end of experiment, rumen liquor of 3 animals of
each treatment (fitted with permanent rumen fistulated) was collected
to determine the concentrations of volatile fatty acids (by gas liquid
chromatography) and NH3-N. Nutrients digestibility were determined
during the last week of the experiment, using chromium, as a marker,
in fecal samples by atomic absorption spectrophotometry. Total digestibility of nutrients, was increased (P < 0.01) by addition of ENZ (ENZ40
and ENZ60) vs control (DM, 65.1 and 69.8 vs 61.7%; CP, 66.1 and 68.9
vs 60.3%; NDF, 50.8 and 54.7 vs 41.7%; ADF, 40.8 and 43.5 vs 32.2%,
respectively). Average daily gain also improved by the addition of the
2 doses of ENZ (1.45 and 1.58 vs 1.25 kg/d, respectively- P < 0.05).
Ruminal fermentation activities were improved by the addition of the 2
ENZ doses, however, VFA were higher (P < 0.05) in ENZ40 and ENZ60
compared with ENZ0 (120 and 128 vs 100 mM/100mL), respectively),
and NH3-N were higher (P < 0.05) in the same treatments vs control
(ENZ0) (65 and 63 vs 54 mg/L, respectively). Supplementing steers
with ENZ could enhance animal performance, ruminal activities and
nutrient digestibility in fattening steers.
increasing levels of an exogenous enzymes preparation (mixture of
cellulase, xylanase, α-amylase and proteases enzymes) in the diet on
ruminal fermentations activates, nutrients digestibility and animal
performance in steers. Forty 8 steers (Baladi × Friesian, 151 ± 0.95 kg
BW) were randomly allocated in 3 experimental groups of the following
treatments (12 steers for each): 0 (control, ENZ0), 40 (ENZ40) and 60 (ENZ60) g of ENZ/head/d. A complete randomize block design was
set up and a repeated measurement in time model used to analyze the
data. The ENZ doses were mixed daily with a part of their daily ration.
Steers were fed a total mixed ration (TMR) contained: 11% cracked
corn, 8.5% agwa (minced date), 26.5% biscuits (bakery waste), 10%
sugar cane molasses, 19% sesame cake, 2% soybean meal, 6% beans,
14.2% rihan straw, 1% salt, 1% limestone and, 0.3 mixture of mineral
and vitamins. Steers were fed the TMR for 206 d as experimental
period. Feed and orts samples were collected twice weekly to calculate
dry matter intake and animals were weighted each 2 weeks during the
experiment. At the end of experiment, rumen liquor of 3 animals of
each treatment (fitted with permanent rumen fistulated) was collected
to determine the concentrations of volatile fatty acids (by gas liquid
chromatography) and NH3-N. Nutrients digestibility were determined
during the last week of the experiment, using chromium, as a marker,
in fecal samples by atomic absorption spectrophotometry. Total digestibility of nutrients, was increased (P < 0.01) by addition of ENZ (ENZ40
and ENZ60) vs control (DM, 65.1 and 69.8 vs 61.7%; CP, 66.1 and 68.9
vs 60.3%; NDF, 50.8 and 54.7 vs 41.7%; ADF, 40.8 and 43.5 vs 32.2%,
respectively). Average daily gain also improved by the addition of the
2 doses of ENZ (1.45 and 1.58 vs 1.25 kg/d, respectively- P < 0.05).
Ruminal fermentation activities were improved by the addition of the 2
ENZ doses, however, VFA were higher (P < 0.05) in ENZ40 and ENZ60
compared with ENZ0 (120 and 128 vs 100 mM/100mL), respectively),
and NH3-N were higher (P < 0.05) in the same treatments vs control
(ENZ0) (65 and 63 vs 54 mg/L, respectively). Supplementing steers
with ENZ could enhance animal performance, ruminal activities and
nutrient digestibility in fattening steers.
Other data
| Title | Effect incremental levels of exogenous enzyme preparation on extent of ruminal fermentation, nutrient digestibilties and average daily gain in steers | Authors | H.M. Gado ; A. Z. M. Salem ; B. E. Borhami | Keywords | beef production;Exogenous enzyme;steers | Issue Date | 2012 | Publisher | Annual meeting of ASAS & ADSA 2012 | Journal | Annual meeting of ASAS & ADSA 2012 |
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