Effect Incremental Levels Of Zado On Cow Milk Production And And Rumen Parameters

Abstract

An experiment was carried out to determine milk production response of Holstein dairy cows to incremental levels of ZADO@ (it is a patent product contains cellulases, xylanases, protease and alpha amylase) in the diet. The experiment was conducted in June and July of 2010. A complete randomize block design was set up and a repeated measurement in time model used to analyze the data. Forty-eight dairy cows were blocked by panty, days in milk, milk production and body weight randomly allocated to one of the following treatments. TO control diet, Tl 40 g of zado cow/day and T2 60 gm of zado/cow/day. Control diet was based on alfalfa plus 5 kg DM of a commercial concentrate (CP: 17.8%, ADF: 17.8%, NDF: 2500 and ASI--I: 7.1%) offered half at each milking. ZADO (cellulose, xylanase, protease, & alpha amylase) was mixed with the concentrate just before each milking session. Cows body weight and milk production at the beginning of the experiment were 574 42, 565+51, 560+40 and 24.2+2.9, 24.6+3.3, 25+2.3, for T0, T1 and T2, respectively. Cows fed ZADO (Tl and T2) produced 1.5 and 2.3 L extra milk, respectively than control cows (TO=24.6 L; P<0.05). Milk production between ZADO fed cows were not significantly different (26. I vs. 26.9 for Tl and T2, respectively. Supplementation of enzymes also increased (P<0.05) rumen ammonia N in comparison to control group. An increase in acetate and propionate at 4 h post-feeding for T1 —T2 (62.0-65.0 and 17.9.3-19.8.8 mol/100 mol respectively; P<0.05) in comparison to control. In conclusion the 40 g ZADO presented the proper inclusion rate of ZADO