EVALUATION OF HISTOPATHOLOGICAL, HISTOCHEMICAL, CYTOLOGICAL AND DNA IMAGE ANALYSIS FINDINGS IN FEMALE ALBINO RATS LIVER TREATED WITH KETOPROFEN

Abstract

The present work is an attempt to clarify the side effects of non-steroidal anti-inflammatory drugs (NSAID) ketoprofen,on the liver.The study was carried out on 96 adult female albino rats weighing 150-200 g,treated orally with ketoprofenat dose levels of 1.34 (therapeutic dose) and 2.67 (over dose) mg/kg body weight for 2months.the histological lesions were in the form of multifocal areas of degeneration in the form of cloudy swelling and fatty changes,inflammatory infiltration in the portal tracts.dilated congested blood vessels and increase in kupffer cells were noticeable within early weeks of low dose administration of ketoprofen and persisted till the end of study.more impressive lesions were perceived with high dose such as wide speard fatty degeneration,dilated sinusoids and bile ducts and lymphocytic accumulation around blood vessels.furthermore,the lesions extended to focal hepatic necrosis scattered throughout the lobules .Proliferation of reticulum fibers were noticed around necrotic areas, enteral veins and portal tracts. Fragments of hemosiderin granules were spotted inside the hepatic tissues. Histochemical staining of total carbohydrate and protein contents revealed a dose and time depended increase .Specific staining for lipids established the increased lipid deposit in the hepatocyte. Marked decrease in acid phosphatase activity in hepatocytes was recorded in all treared animale .Likewise , marked elevation of hepatic alkaline phosphatase activity in earlier weeksssss was followed by decrease in later weeks . Cytological examination is a useful method for rapid accurate diagnosis. The changes noticed in cytological imprints paralleled those in histologic sections, denoting the high diagnostic yield of cytologic diagnosis .Accurate measurement of the DNA content in the hepatocytes to measure the DNA ploidy using Feulgen stain of liver imprint and by use of the DNA image analyser system was done .It illustrated the normal hepatocytes content of DNA (2C) and does not reach to(5C) . This confirms the absences of any aneuploidy in the liver cells. Thus, we can conclude that NSAIDs administration can be associated with histopathological changes in the liver aneuploidy of the cells were not encountered.