Evaluation of Circular RNA Network Expression in Hepatocellular Carcinoma
Esraa Mohamed Ali;
Abstract
Serum sample was taken for exosomal RNA extraction and real-time polymerase chain reaction (qPCR) for measuring mRNA-AAK1, hsa_circ_002136 and miRNA-106b level. The data were normalized using the endogenous ACTB and RNU U6 as reference control. The threshold cycle (Ct) value of each sample was calculated with Step One PlusTM software v2.2.2 (Applied Biosystems), and the 2- ΔΔCT method was used in the analysis of PCR data for relative quantification of mRNA-AAK1, hsa_circ_002136 and miRNA-106b.
The results were calculated and statistically analyzed by the SPSS software.
Participants were excluded from the study if Patients actively undergoing chemotherapy or radiation therapy for HCC or patients diagnosed with other malignancies or treated within the last 5 years.
The results were calculated and statistically analyzed by the SPSS software.
Participants were excluded from the study if Patients actively undergoing chemotherapy or radiation therapy for HCC or patients diagnosed with other malignancies or treated within the last 5 years.
Other data
| Title | Evaluation of Circular RNA Network Expression in Hepatocellular Carcinoma | Other Titles | تقييم مستوى شبكة الأحماض الريبوزية النووية الدائرية فى مرضى سرطان الكبد | Authors | Esraa Mohamed Ali | Issue Date | 2018 |
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