CpG Island Hypermethylation in Kallikrein-Related Peptidase 10 Gene (KLK10) as a Clue for Ovarian Tumor Biology, Diagnosis and Prognosis
Mustafa Abdel Hafiz El Sherbini;
Abstract
In the present study we assessed the methylation of KLK10 exon 3 using methylation-specific PCR (MSP). The included samples were archival FFPE-ovarian tissue samples that are available at the Early Cancer Detection Unit, Ain Shams University Maternity Hospital. The studied ovarian tissue samples were 16 benign and 16 malignant tumors. In addition, 16 normal samples were included as controls.
The experimental protocol was: 1) DNA extraction using commercially available DNA extraction kit for FFPE-tissue, 2) DNA Bisulfite conversion using commercially available DNA Bisulfite conversion kit and 3) MSP using HotStarTaq Master Mix kit. The PCR products were assessed by conventional 2% agarose gel electrophoresis and capillary gel electrophoresis.
The experimental protocol was: 1) DNA extraction using commercially available DNA extraction kit for FFPE-tissue, 2) DNA Bisulfite conversion using commercially available DNA Bisulfite conversion kit and 3) MSP using HotStarTaq Master Mix kit. The PCR products were assessed by conventional 2% agarose gel electrophoresis and capillary gel electrophoresis.
Other data
| Title | CpG Island Hypermethylation in Kallikrein-Related Peptidase 10 Gene (KLK10) as a Clue for Ovarian Tumor Biology, Diagnosis and Prognosis | Other Titles | دراسة منسوب مجموعة الميثيل في جين الببتيداز المنتسب للكاليكرين 10 كدليل محتمل في تطور و تشخيص و علاج سرطان المبيض | Authors | Mustafa Abdel Hafiz El Sherbini | Issue Date | 2018 |
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