PHYTOCHEMICAL AND BIOLOGICAL STUDIES OF Terminalia laxiflora LEAVES GROWING IN EGYPT

Abstract

In this the study, Terminalia laxiflora leaves were used to identify the active substances in it and to determine antimicrobial and antioxidant effects. T. laxiflora tree belonging to the family Combrtaceae is cultivated in Egypt. Extract of T. laxiflora leaves as aqeous methanolic, methylene chloride, ethyl acetate and butanol were tested for thier antioxdant activity using DPPH free radical scavenging assay. Moreover, the phytochemical screening for qualitative analysis of phenolic constituent were evaluated using KIO3 test for gallotannins, NaNO2 test for ellagitannins, Shinoda test for flavonoids and FeCl3 test for phenolics. In general, Terminalia laxiflora methanolic extract and ethyl acetate extract showed the most highest antioxidant activity of IC50 =9.86 and IC50 =10.13; respectively with highest phenolic constituents. Therefore, Terminalia laxiflora methanolic extract and ethyl acetate extract should be used as a better radical scavenging agents. The HPLC analysis for phenolic compounds of methanolic extract of Terminalia laxiflora showed gallic acid with the highest level followed by p-hydroxybenzoic acid, then sinapic acid and rosmarinic acid, but the lowest amounts were obsereved for cinnamic acid and caffiec acid