PROPAGATION OF THE RARE ORNAMENTAL CLIMBER Porana panculata BY TISSUE CULTURE

Abstract

During the establishment stage, explants exposed to Clorox at 20% for 15 min obtained the highest position in survival %. Using 2,4-D at 1.0 ppm + BAP at 2.5 ppm occupied the 1st position in callusing %, callus fresh weight and callus diameter. Applying 2-iP at 3 ppm + 1/2 MS medium produced the highest values of callus survival %, morphogenic callus %, culture efficiency, shoot number, shoot length and leaf number. In the multiplication stage, using BAP at 0.60 ppm + 1/2 MS medium resulted in the highest records of lateral buds survival %, regeneration capacity, culture efficiency, shoot number, shoot length and leaf number. BAP at 1 ppm + NAA at 0.1 ppm recorded the 1st position concerning shoot survival %, shoot number, shoot length and leaf number. Concerning the rooting stage, the 1st position for survival %, root number and shoot number was obtained when combinations of BAP plus IBA at either 1 or 3 ppm were used