THE EFFECT OF VITAMIN E AND C ON THE FREE RADICAL DEFENSE SYSTEM AND INSULIN SENSITIVITY OF EXPERIMENTAL ANIMALS FED HIGH FRUCTOSE DIET

Abstract

Seventy two growing male red hamesters 8 weeks old weighing between 100-110 grn were divided into 8 groups, nine hamesters; each one of them was fed on semipurified diet (casein diet) as negative control (group 1) while the second group was fed on high carbohydrate diet containing 33.6% of fructose as positive control (group 2). Three groups of the remaining were fed on a high fructose diet supplemented with antioxidants vitamins [C, E and C combined withE (groups 3, 4 and 5)]. Group 6 fed a fructose diet supplemented with 2.5 gm vitamin C/kg diet, group 7 which fed a diet contained 500 • mg vitamin E as (a-tocopherol)/kg diet and group 8 which fed a diet contained a combination of2.5 gm vitamin Cwith 500 mg vitamin E/kg diet. The other three groups (3, 4 and 5) were received a semipurified diet (casein diet) supplemented with 2.5 gm vitamin C/kg diet, 500 mg vitamin E (a-tocopherol)/kg diet and a combination of2.5 gm vitamin C with 500 mg vitamin E/kg diet respectively. The duration of the treatment were 8 wk, at the end of these period all hamesters were scarified. Plasma and whole bloGd samples were collected in clean heparinized tubes. Liver, kidney and spleen were removed and kept directly in the freezer at (-l8°C) until analysis. Total lipids was determined in liver.