Studies on Lumpy Skin Disease Virus Vaccines

Abstract

In this work there were 3 experiments, the firstwas done on 10 pregnant cows vaccinated•. with• SPV vaccine at different stages,of pregnancy, and boo3tered at gth month of pregnancy. The results explaiiied ..

that the vaccinated cows at first stage of pregnancy (1st, 2nd & 3rd month) gave hi.gh neutralizing antibody titers after boostering, while cows which vaccinated at middle stage of pregnancy and also boostering at 8th month of pregnancy gave less neutralizing antibody titers.

The second experiment was applied on calves vaccinated with SPV vaccjne at different ages, it was found that the maternal immunity still up to 7 month of age and the best time of vaccination to calves 6-7 month age where after this the results of VNT and ELISA gave high titers.

- Propagation of LSDV (Ismailia 88 strain) on MDBK cell culture to obtain a high titer 7.5 log10TCID50 I rnl after 6th passage. - Inactivation of LSDV by BEl 2 %occurred after 12 hours.

-Inactivated LSDV gave nq CPE on MDBK cell culture, and no symptoms

I on susceptible animals which inoculated with it. -Inactivated LSDV was adjuvanted by N.sativa oil. I -The prepared inactivated N.sativa oil adjuvant LSDV was found completely sterile and free from any contaminants.

-Safety test of prepared vaccine was checked by the S/C inoculation of 10 I ml into susceptible calf. No symptoms or death or postvaccinal reaction were recorded during the incubation period (one week) or rise in I temperature. I