Phenotypic screening and molecular characterization of carbapenem resistance of certain pathogenic Gram negative bacterial isolates in Egypt
Noha Ahmed Kamel Ahmed;
Abstract
Abstract
Globally, carbapenem resistance is a major on-going public health problem that mainly occurs among Gram negative pathogens such as Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa) and Acinetobacter baumannii (A. baumannii). Carbapenem resistance may be intrinsic or mediated by transferable carbapenemase encoding genes. Accordingly, the aim of the current study was to characterize the prevalence of carbapenemase producing Gram negative bacilli recovered from children with malignancy in Egypt. Standard method were used for culture identification, sensitivity testing (Kirby Bauer and broth microdilution method) and carbapenemase detection. A total of 187 Gram negative bacilli (GNB) were recovered from different clinical specimens, E. coli (88; 47%), followed by Klebsiella spp. (72; 38.5%) and A. baumannii (7; 3.74%). It is a matter of concern that 162 out of 172 enterobacterial isolates (94.18%) showed resistant to three or more antimicrobial classes and were considered multidrug resistant. Additionally, the rate of carbapenem resistance displayed a worrisome trend as 114 out of 172 enterobacterial isolates (66.27%) and 11 out of 13 non fermenting Gram negative bacilli (84.61%) were resistant to at least one of the tested carbapenems by disk diffusion method. After performing a series of phenotypic tests as initial screening for potential carbapenemase producers, molecular characterization to the 29 extracted plasmids were subjected to multiplex PCR (using 5 common carbapenemase primers). The results reveal that blaOXA-48 was the most prevalent 17 (58.62%), followed by blaNDM 8 (27.58%), then blaVIM 3 (10.3%) and blaKPC 2 (6.89%). These results are alarming threat to public health that calls for urgent application of antimicrobial stewardship programs along with routine surveillance for controlling outbreaks.
Globally, carbapenem resistance is a major on-going public health problem that mainly occurs among Gram negative pathogens such as Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa) and Acinetobacter baumannii (A. baumannii). Carbapenem resistance may be intrinsic or mediated by transferable carbapenemase encoding genes. Accordingly, the aim of the current study was to characterize the prevalence of carbapenemase producing Gram negative bacilli recovered from children with malignancy in Egypt. Standard method were used for culture identification, sensitivity testing (Kirby Bauer and broth microdilution method) and carbapenemase detection. A total of 187 Gram negative bacilli (GNB) were recovered from different clinical specimens, E. coli (88; 47%), followed by Klebsiella spp. (72; 38.5%) and A. baumannii (7; 3.74%). It is a matter of concern that 162 out of 172 enterobacterial isolates (94.18%) showed resistant to three or more antimicrobial classes and were considered multidrug resistant. Additionally, the rate of carbapenem resistance displayed a worrisome trend as 114 out of 172 enterobacterial isolates (66.27%) and 11 out of 13 non fermenting Gram negative bacilli (84.61%) were resistant to at least one of the tested carbapenems by disk diffusion method. After performing a series of phenotypic tests as initial screening for potential carbapenemase producers, molecular characterization to the 29 extracted plasmids were subjected to multiplex PCR (using 5 common carbapenemase primers). The results reveal that blaOXA-48 was the most prevalent 17 (58.62%), followed by blaNDM 8 (27.58%), then blaVIM 3 (10.3%) and blaKPC 2 (6.89%). These results are alarming threat to public health that calls for urgent application of antimicrobial stewardship programs along with routine surveillance for controlling outbreaks.
Other data
| Title | Phenotypic screening and molecular characterization of carbapenem resistance of certain pathogenic Gram negative bacterial isolates in Egypt | Other Titles | الفحص الظاهري والتوصيف الجزيئي لمقاومة الكربابينيم في بعض العزلات البكتيرية الممرضة سالبة الجرام في مصر | Authors | Noha Ahmed Kamel Ahmed | Issue Date | 2018 |
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