Biological and Green Synthesis of Some Nanoparticles Having Biofouling Inhibitory Effect

Abstract

• Different microbial strains were tested for their ability to reduce different metal salts to synthesize NPs including; bacteria, fungi and yeast. In this study proved that Aspergillus brasiliensis ATCC 16404 mycelial cell free filtrate (MCFF) showed good potential for the biosynthesis of silver nanoparticles (AgNPs). One-factor-at a time-optimization technique (OFAT) was applied to optimize the mycosynthesis of AgNPs, which revealed maximum production of 0.17 g AgNPs using 100 ml of the MCFF of 6 % (w:v) A. brasiliensis biomass and 2 mM silver nitrate (AgNO3) at pH 7, under fluorescent light static incubation for 72 h at 20oC. The mycosynthesized mono-dispersed spherical shaped AgNPs of average size 6-21 nm proved to be stable for about 60 days. • Cobalt oxide nanoparticles (Co3O4-NPs) were successfully mycosynthesized for the first time by A. brasiliensis ATCC 16404 MCFF. The preliminary indication for the formation of Co3O4-NPs was the change in color from yellow to reddish color. OFAT technique was applied to determine the optimum physicochemical conditions required for the mycosynthesis of Co3O4-NPs. That were found to be; 72 h for reaction time, 11 for pH