BIOCHEMICAL BEHAVIOUR OF 14C LABELED POLYAMINES IN SOME FABA BEAN GENOTYPES UNDER RAINFED AT MARYOUT CONDITIONS
NOURA EBRAHIM MAHMOUD;
Abstract
The expansion of the new reclaimed land should be carried out through a new concept of rationalization of water, utilization of rainfall areas, use of tolerant genotypes and modern applications to induce plants to water stress tolerance. Therefore, this study was performed to assess the contribution of exogenous polyamine precursors (arginine and methionine at values 20, 40 and 60 ppm) as well as polyamines compounds (putrescine, spermidine and spermine at concentrations of 0.5, 1 and 1.5 μM) for water stress tolerance in two faba bean cultivars with supplementary irrigation under rainfall conditions at Maryout.
The results showed a clear superior of Maryout2 cultivar in seeds and straw yield compared to Nubaria1 cultivar. Also, treatment with polyamines and precursors had positive effects on yield and some chemical components, a clear increase in seeds and straw yield was observed when Maryout2 was treated with arginine and methionine at value 60 ppm as well as putrescine and spermidine at rate of 1 μM compared with the control and associated with a decrease in the content of malondialdehyde and hydrogen peroxide. In the same direction, spermine at rate of 1.5 μM had a positive effect on characteristics of Nubaria1 cultivar. The results showed accumulation in pigments, endogenous free amino acids and polyamines, activation of antioxidant enzyme such as superoxide dismutase and peroxidase and soluble protein content in faba bean cultivars.
Also, this study was conducted to find out the vital and effective role of arginine in polyamines biosynthesis for faba bean. Two cultivars were treated with 60 ppm 14C-arginine after 35 days from cultivation. Plant samples were done at consecutive time periods and distribution of the 14C- in two cultivars. Data observed the quantity of 14C- on surface of faba bean leaves were gradually absorbed and decreased from leaves to inter into plant cells. Maryout2 cultivar has characterized the ability to absorb of 14C- rapidly than Nubaria1 cultivar and subsequent rapid metabolic process. Also, the overall ratio of 14C-arginine is almost constant in two cultivars throughout the experiment. However, there difference in distribution of 14C- in two arginine forms (free and combined) whereas 14C-accumulation in combined form more than free in two cultivars. In the same time, 14C- was accumulation in toluene layers more than aqueous layers resulting from polyamines extract. Similar trend, 14C- accumulated in polyamines fractions (putrescine, spermidine, spermine and cadaverine). The accumulation of 14C- in putrescine in free form more than other fractions in two cultivars while 14C- was accumulated by Maryout2 more than Nubaria1. 14C- was accumulated in spermidine and putrescine fraction more than other fractions in soluble and insoluble conjugate polyamines forms in Maryout2 respectively
The results showed a clear superior of Maryout2 cultivar in seeds and straw yield compared to Nubaria1 cultivar. Also, treatment with polyamines and precursors had positive effects on yield and some chemical components, a clear increase in seeds and straw yield was observed when Maryout2 was treated with arginine and methionine at value 60 ppm as well as putrescine and spermidine at rate of 1 μM compared with the control and associated with a decrease in the content of malondialdehyde and hydrogen peroxide. In the same direction, spermine at rate of 1.5 μM had a positive effect on characteristics of Nubaria1 cultivar. The results showed accumulation in pigments, endogenous free amino acids and polyamines, activation of antioxidant enzyme such as superoxide dismutase and peroxidase and soluble protein content in faba bean cultivars.
Also, this study was conducted to find out the vital and effective role of arginine in polyamines biosynthesis for faba bean. Two cultivars were treated with 60 ppm 14C-arginine after 35 days from cultivation. Plant samples were done at consecutive time periods and distribution of the 14C- in two cultivars. Data observed the quantity of 14C- on surface of faba bean leaves were gradually absorbed and decreased from leaves to inter into plant cells. Maryout2 cultivar has characterized the ability to absorb of 14C- rapidly than Nubaria1 cultivar and subsequent rapid metabolic process. Also, the overall ratio of 14C-arginine is almost constant in two cultivars throughout the experiment. However, there difference in distribution of 14C- in two arginine forms (free and combined) whereas 14C-accumulation in combined form more than free in two cultivars. In the same time, 14C- was accumulation in toluene layers more than aqueous layers resulting from polyamines extract. Similar trend, 14C- accumulated in polyamines fractions (putrescine, spermidine, spermine and cadaverine). The accumulation of 14C- in putrescine in free form more than other fractions in two cultivars while 14C- was accumulated by Maryout2 more than Nubaria1. 14C- was accumulated in spermidine and putrescine fraction more than other fractions in soluble and insoluble conjugate polyamines forms in Maryout2 respectively
Other data
| Title | BIOCHEMICAL BEHAVIOUR OF 14C LABELED POLYAMINES IN SOME FABA BEAN GENOTYPES UNDER RAINFED AT MARYOUT CONDITIONS | Other Titles | السلوك الكيميائي الحيوي لعديد الأمينات المرقمة بالكربون 14 في بعض التراكيب الوراثية للفول البلدي تحت الظروف المطرية بمريوط | Authors | NOURA EBRAHIM MAHMOUD | Issue Date | 2019 |
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