Treatment of different types of cancer cells using bacterial L-asparaginase
Noha Emad El Din Abdel Razek;
Abstract
Abstract
In this study, two L-asparaginase producing bacteria were selected among 53 isolates and identified by Biolog identification system as Pectobacterium carotovorum and Serratia marcescens. Optimization increased the production of L-asparaginase from 4.497 U/ml and 4.238 U/ml to 4.835 and 5.221 U/ml for P. carotovorum and S. marcescens respectively. L-asparaginase was partially purified and tested in vitro for cytotoxic activity using MTT (3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay against MCF-7, HepGII and WISH cell line. L-asparaginase from P. carotovorum and S. marcescens were not cytotoxic to normal epithelial WISH cells. On the other hand, L-asparaginase from both isolates was cytotoxic to MCF-7 and HepGII cancer cell lines with an IC50 of (15 μg/ml and 26 μg/ml) and (26 μg/ml and 25 μg/ml) respectively. The expression for the regulatory genes; BCl2, Bax and P53 in L-asparaginase treated cancer and normal cell lines were analyzed by RT-PCR. High expression of BCl2 and P53 gene in MCF-7, HepGII and WISH cell line was observed. In addition, the cytotoxicity induced by L- asparaginase from P. carotovorum and S. marcescens on both cancer cell line MCF-7 and HepGII was associated with the expression of BCl2 and P53 indicated the activation of apoptotic pathway.
In this study, two L-asparaginase producing bacteria were selected among 53 isolates and identified by Biolog identification system as Pectobacterium carotovorum and Serratia marcescens. Optimization increased the production of L-asparaginase from 4.497 U/ml and 4.238 U/ml to 4.835 and 5.221 U/ml for P. carotovorum and S. marcescens respectively. L-asparaginase was partially purified and tested in vitro for cytotoxic activity using MTT (3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay against MCF-7, HepGII and WISH cell line. L-asparaginase from P. carotovorum and S. marcescens were not cytotoxic to normal epithelial WISH cells. On the other hand, L-asparaginase from both isolates was cytotoxic to MCF-7 and HepGII cancer cell lines with an IC50 of (15 μg/ml and 26 μg/ml) and (26 μg/ml and 25 μg/ml) respectively. The expression for the regulatory genes; BCl2, Bax and P53 in L-asparaginase treated cancer and normal cell lines were analyzed by RT-PCR. High expression of BCl2 and P53 gene in MCF-7, HepGII and WISH cell line was observed. In addition, the cytotoxicity induced by L- asparaginase from P. carotovorum and S. marcescens on both cancer cell line MCF-7 and HepGII was associated with the expression of BCl2 and P53 indicated the activation of apoptotic pathway.
Other data
| Title | Treatment of different types of cancer cells using bacterial L-asparaginase | Other Titles | معالجة انواع مختلفة من الخلايا السرطانية بإستخدام الاسباراجيناز البكتيري | Authors | Noha Emad El Din Abdel Razek | Issue Date | 2019 |
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