Relation of Genetic Polymorphism of ABCG2 Gene and Occurrence of Oxaliplatin- Induced Peripheral Neuropathy in Patients with Gastrointestinal Tract Cancer

Abstract

Background: Oxaliplatin use in gastrointestinal malignancies is limited by neurotoxicity. The aim of the current study was to identify single-nucleotide polymorphisms (SNPs) in ABCG2 gene involved in oxaliplatin-induced peripheral neuropathy (OIPN) among Egyptian Colorectal Cancer (CRC) patients treated with oxaliplatin-based chemotherapy (CT) together with its association with other patient characteristics. Patients and methods: All eligible Colorectal Cancer patients were included in the study if they were between 18-80 years old and excluded if they had a neurologic disease or a disease impairing neurologic function. OIPN was evaluated and graded on day 1 of each CT cycle according to the National Cancer Institute Common Terminology Criteria for Adverse Events (NCI- CTCAE v.4). The Genomic DNA was extracted from blood to detect SNPs within the ABCG2 gene (at rs2231137and at rs3114018) using High Resolution Melting (HRM) technique followed by direct sequencing method for every melting cluster using 3500 genetic analyzer for the samples selected from each cluster.