“The Effect of Hemostatic Agents Contamination on the Bonding Performance of Different Universal Adhesives: An in vitro comparative study”

Abstract

This study was conducted to evaluate and compare the bonding performance regarding: bond strength, nanoleakage and resin/ dentin interface of three universal adhesives when applied on bovine dentin after either no contamination or after contamination with two different types of hemostatic agents and after cleaning the dental surface with either water , phosphoric acid or EDTA. For the micro-shear bond strength test, a total of 135 bovine permanent anterior teeth extracted from sacrificed bovine jaws were used. They were divided into three main groups (n=45) according to the Universal adhesive used (Single bond universal, Futurabond M+, Tetric N bond Universal). Each group was further divided into three subgroups (n=15) according to the hemostatic agents used (No hemostatic agent, ViscoStat Clear or Visine Original). And each subgroup was divided into three classes (n=5) according to the cleaning method used (water, phosphoric acid or EDTA). Micro-shear bond strength testing was done using Universal Testing Machine, and the test was run at a crosshead speed of 0.5mm/min until failure. The µSBS in MPa was calculated by dividing the load (Newton) over the respective surface area (mm2).