PHYSIOLOGICAL AND IMMUNOLOGICAL STUDIES ON EXPERIMENTAL ENTAMOEBIASIS
Safaa Moustafa Taha Hassan;
Abstract
Amoebiasis is a common endemic disease in Egypt and in many countries sharing the same hygienic conditions. It is characterized by a chronic course, where the infection may spread from the intestine to liver, lungs and brain. This leads to death due to dangerous complications. Recent interest has been focused on
•
studying the severity of the infection by serological diagnosis.
The present study was , therefore, designed to study the pattern of the infection by the parasitological examio.ation of the stool, and
I
estimate the total leucocyte count in addition to evaluate the
I
I
antibody response at the different parasitolbgical manifestations,
i
1
compared with uninfected control group .
The present work was carried out oh 300 free parasitic
experimental mice . These divided into three groups, the first group
I
received 50 Entamoeba histolytica cysts obtained from carrier
amoebic case. The second group inoculated• with 50 E.histolytica cysts obtained from chronic amoebic case. The third group kept away from infection as uninfected control group.
'
The pattern of infection was studied in 'cyst passers cases by
counting the excreted cysts daily up to one month. The number of cysts were increased gradually, reaching the maximum number at days 14 to 16 after infection, then declined graduaily, reaching the minimum at day 28 after infection, being zero at day 30 after
infection. In trophozoite cases, trophozoites counting were done
weekly, up to one month. The maximal count oftrophozoites was at
. I
day 21 after infection, and still relatively. high during the fourth
week after infection.
Total leucocytic count were done weekly for infected cases and compared with control group. In cyst passers cases, a significant increase in total leucocyte count reaching 5,660 and 5,760 WBCs were found during the third and the fourth weeks after infection, respectively compared with control group. While the increase was insignificant during the first and the second weeks after infection. A significant elevation in total leucocyte count in trophozoite cases during the four weeks of the experiment reaching, 6,550, 6,990,
9,120 and 9,410 WBCs , respectively. The total leucocyte count
in control group ranging from 4,980 to 5,360 WBCs .
•
studying the severity of the infection by serological diagnosis.
The present study was , therefore, designed to study the pattern of the infection by the parasitological examio.ation of the stool, and
I
estimate the total leucocyte count in addition to evaluate the
I
I
antibody response at the different parasitolbgical manifestations,
i
1
compared with uninfected control group .
The present work was carried out oh 300 free parasitic
experimental mice . These divided into three groups, the first group
I
received 50 Entamoeba histolytica cysts obtained from carrier
amoebic case. The second group inoculated• with 50 E.histolytica cysts obtained from chronic amoebic case. The third group kept away from infection as uninfected control group.
'
The pattern of infection was studied in 'cyst passers cases by
counting the excreted cysts daily up to one month. The number of cysts were increased gradually, reaching the maximum number at days 14 to 16 after infection, then declined graduaily, reaching the minimum at day 28 after infection, being zero at day 30 after
infection. In trophozoite cases, trophozoites counting were done
weekly, up to one month. The maximal count oftrophozoites was at
. I
day 21 after infection, and still relatively. high during the fourth
week after infection.
Total leucocytic count were done weekly for infected cases and compared with control group. In cyst passers cases, a significant increase in total leucocyte count reaching 5,660 and 5,760 WBCs were found during the third and the fourth weeks after infection, respectively compared with control group. While the increase was insignificant during the first and the second weeks after infection. A significant elevation in total leucocyte count in trophozoite cases during the four weeks of the experiment reaching, 6,550, 6,990,
9,120 and 9,410 WBCs , respectively. The total leucocyte count
in control group ranging from 4,980 to 5,360 WBCs .
Other data
| Title | PHYSIOLOGICAL AND IMMUNOLOGICAL STUDIES ON EXPERIMENTAL ENTAMOEBIASIS | Other Titles | دراسة فسيولوجية مناعية لعدوى الدوسنتارييا الاميبية فى حيوانات التجارب | Authors | Safaa Moustafa Taha Hassan | Issue Date | 1998 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| B13708.pdf | 1.04 MB | Adobe PDF | View/Open |
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