MORPHOMETRIC AND ULTRASTRUCTURAL CHANGES OF TESTIS IN DIABETIC RATS

Abstract

Diabetes is a complicated syndrome that affects the anatomical structure and function of many organs. Infertility is a problem that results from many factors including diabetes. The aim of this work is to evaluate . the effect of diabetes on the rat testis. MATERIALS AND METHODS 45 albino rats weighing from 200-250 gm were used in this study and were divided into two main groups. Group I (Control non diabetic) Composed of 15 rats kept without any treatment. They served as control for treated groups, all the time of experimental period ( three months). Group II (Experimental diabetic) Composed of 30 rat injected by alloxan to 'induc e diabetes by single intraperitoneal injection in dose 150 mg!kg. Animals were sacirfied at one, two and three months after induction of diabetes. SpeciJnens from the testis • were carefully dissected out and processed for paraffm blocks, morphomtric and electron microsopic studies. HISTOLOGICAL EXAMINATION Group I (control non diabetic) 'I Control rats showed the well known histological picture of rat testis. •.', It consists of rounded to oval seminiferous tubules. Each tubule is bounded by a basal lamina and lined by spermatogenic and Sertoli cells. In between the tubules there is a testicular intestitium containing fibroblasts, collagen, blood and lymphatic vessels together with clumps of Leydig cells.