Photophysical studies and photoanalytical techniques for assessment of some important compounds

Abstract

Physical studies and photoanalytical techniques for assessment of some important compounds The study presented in this thesis includes development and the combination between some techniques and technologies such as Analytical chemistry, Biochemistry, Photochemistry and Nanotechnology for the determination of some important compounds such as Alpha amylase Enzyme, Cortisol Hormone and Nalbuphine Drug in the human serum.

The thesis is divided into four chapters:

Chapter One:- Includes a general introduction on lanthanides, optical sensors and other methods used for determination of Alpha amylase Enzyme, Cortisol Hormone and Nalpuphin in the human serum .

Chapter Two:- Describes a new nano-optical sensor Tb (III) - carboxyphenyl-18-Crown-6 complex doped in sol-gel matrix for assessment of Alpha amylase activity in human serum and using the alpha amylase as biomarker for the early diagnosis of pancreatitis & parotitis for human. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated. Under optimal conditions, the luminescence intensity of Tb3+- carboxyphenyl-18-crown-6 doped in sol gel matrix is inversely proportional to the concentration of 2-CNP in range ( 3.0 X10-4 – 8.0 X 10-9) mol L−1, ( r = 0.999 ). The detection limit(LOD) (S:N=3) and quantitation limit of detection (LOQ) are (1.2 x 10-9 and 3.6 x 10-9) mol