Novel nano optical sensors for the assessment of some materials of biological importance in pharmaceutical & serum samples

Abstract

The preparation and characterization of new optical sensors for assessment of some material of biological importance The aim of the present work is the development and introduction of modern analytical techniques with high sensitivity and selectivity with low cost for diagnosis for liver cirrhosis by assessment of 3-Nitrotyrosine in serum and assessment of amirolide HCl in serum and urine samples. The study is divided into 3 chapters: Chapter one Includes a general introduction on lanthanides and other methods used for assessment of 3-Nitrotyrosine in serum and assessment of amirolide HCl in serum and urine samples. Chapter two Describes highly selectivity and sensitivity spectrofluorimetric method for assessment of 3-Nitrotyrosine as a biomarker for early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE) disease based on quenching the luminescence intensity of the optical sensor (Eu(TTA)3Phen) after addition of different concentrations of 3-Nitrotyrosine in DMSO at pH=9 and λex=375nm, making linear relation between fluorescence intensity against inverted concentration and making wide linear working range (8.8 x 10-6 - 3.4 x 10-9 ) mol L-1 and the limit of detection (LOD) and quantitation limit of detection (LOQ) are (1.6 x 10-9 and 4.9 x 10-9 mol L-1).