Use of Some Molecular Techniques for Detection of Foot and Mouth Disease Virus in Sheep and Goats

Abstract

A total of 471 samples of Sheep and Goats samples were collected from the governorates of El-Beheira, Sharqia, Kafr El-Sheikh and Minya. The ELISA solid phase test was used to detect antibodies to FMD and the FMD ELISA was used to detect antibodies to FMD Against non-structural protein. A total of 87 field samples were collected from sheep and goat cases showing symptoms of foot and mouth disease from Alexandria, Dakahlia, El-Gharbia, Menoufia, Qalubia, Cairo, Suez, Ismailia, Fayoum, and El Menia governorates. The virus was isolated by three consecutive times on the total cells of the BHK, where 16 samples showed a cytopathic effect in the cells within 24-48 hours after injection. The results of the identification of 87 samples using the Indo Sandwich Elisa test showed that there were 33 samples Positive The real-time polymerase reaction was used to isolate 16 isolates and gave positive results. The virus was diagnosed in 12 samples using serial polymerase reaction, preceded by reverse transcription to polymerase the protein gene of 2B using general primers. It was introduced with special triggers for type O and type A. The genetic sequence of 3 positive samples of type O and 3 species samples (A) and put it with the known genetic species to create a causative tree.