STUDY OF DNA CONTENT AND MORPHOMETRIC FEATURES OF URINARY BLADDER SQUAMOUS AND TRANSITIONAL-CELL CARCINOMA

Abstract

In this study, image cytometric analysis of nuclear DNA content and morphometric features was performed on (90) specimens of urinary bladder . carcinomas; (60) squamous cell (Sq.C.C) and (30) transitional cell carcinomas (T.C.C), in addition (10) urinary bladder controls. In urinary bladder Sq.C.C 61.7o/o were associated with schistosomiasis. There was male predominance in

almost all studied cases, the mean age for Sq.C.C was 55yr and was 60yr for T.C.C.

Regarding the DNA content analysis, all controls were diploid but in cases ofSq.C.C and T.C.C, there is increasing of aneuploidy with increasing of grades. The SPF, Sc exceeding rate and 2 CDI were increased step-wise from grade!to grade 3 with significant difference. The aneuploidy difference between schistosoma! and non­ schistosomal Sq.C.C was insignificant. The morphometric parameters for nuclear size as mean nuclear area, perimeters and diameters showed progressive increase from grade I to grade 3 Sq.C.C and T.C.C. The morphometric parameters for nuclear shape as nuclear form factor, nuclear contour index and nuclear ovality were statistically insignificant to differentiate grades and stages of urinary bladder carcinoma.