Clinical and Histopathological Assessment of the Healing Potential of Periapical Lesions Using Different Protocols

Abstract

The aim of the present study was to detect the role of Platelet Rich Fibrin, synthetic bone graft and triple antibiotic paste in healing and regeneration of periapical tissues following periapical surgery. The study included clinical, radiographic and histopathologic changes in dogs. This study included six adult Canaan dogs of both sexes. The total number of teeth (96) in 6 dogs (16 tooth per dog). In each dog the sixteen teeth were divided randomly into four equal groups (4 teeth each group) according to treatment protocol. After general anesthesia all teeth were examined radiographically (periapical radiographs) to confirm complete root formation, to estimate the length of the roots and to establish base line periapical tissues for further comparison of the periapical area. For these groups, after induction of periapical lesion and root canal obturation, apical surgery of teeth was performed and the created bony defect was filled in the three experimental groups and it was left empty in the control group. The dogs were anaesthesized. Full thickness mucoperiosteal flaps were performed. Proper reflection and elevation of the flaps were done.