Effect of different irrigation protocols on Transforming growth factor β1 release from dentin matrix and its relation to pulp regeneration (In –vivo Study)

Abstract

The aim of this study was to evaluate the effect of different irrigation protocols on the release of Transforming growth factor β1 from the dentin matrix and the regenerative potential of stem cells. A total of 48 human permanent single rooted anterior teeth were used in this study. Decoronation was done, two discs from each root were obtained for each evaluation part of the experiment and randomly allocated to 5 groups with different irrigation protocols. Part I: Evaluation of TGF-β1 release: The discs were kept at 37º C for 1 day and at the end of the time point, medium from samples was collected and filtered. The solution was removed, immediately frozen in liquid nitrogen, and stored at -80ºC. After completion of sample collection, all samples were thawed and subjected to growth factor quantification by using an ELISA test system for TGF-β1.