ROLE OF TRICHINELLA SPIRALIS EXCRETORY /SECRETORY PRODUCTS IN THE DEVELOPMENT OF MICE RESISTANT TO NFECTION

Abstract

The vaccine research always has to compete with the development of anthelmintics. Whereas the latter increasingly cover a broad range of endo­ and even ectopar sites, vaccines are efficient against only one parasite species. However, with increasing resistance of parasites against anthelmintics and ecological awareness about drug residues, vaccines become more and more important. Because of its zoonotic importance and lacking prophylactic strategies a vaccine against T. spiralis would be of a great advantage. Excretory/secretory products are considered very important from an immunological point of view as they are easily targeted by the immune system. The present work aimed to show the role of excretory/secretory products (antigens) of adult and larval stages T. spiralis in causing protection or induction of the host immune response. Adult and larval ES products were prepared by in vitro cultivation of these stages under aseptic conditions, %5 C02 and 37°C. The activity of acetylcholinesterase and superoxide dismutase isozymes, which are considered as immune targets were characterized in both preparations of the ES products by the electrophoretic technique. The ES antigens ofT. spiralis adult and larval stages were orally adminsterd

to two groups of mice 300 ug/ml each, divided to three doses weakly without adjuvant, and then challenged with 150 T.spiralis infective larvae one week after the last immunizing dose. Another experimental group was challenged with 150 larvae and served as infected control. The experimental groups were dissected on days 7,14,21,28 and 35 post challenge infection, to clarify the following items: