COMPARATIVE GENOMIC STUDIES OF SOME LAMIACEAE FAMILY PLANTS
HADER MOHAMED AHMED AHMED MANSOUR;
Abstract
This study was carried out at the Molecular Cytogenetic Laboratories and Molecular genetics Laboratory, Department of Genetics, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Qaliubiya, Egypt, during the period from 2015 to 2020.
In our study, six local Egyptian commercial cultivars of family lamiaceae were studied, this family is one of the three largest family that contain Herbal medicines (Asteraceae, Rosaceae and Lamiaceae) that have used from ancient times. Two regions sequences were analyzed to compare between species, the first region was ribulose 1,5-bisphosphate carboxylase Large (rbcL) gene at the level of DNA sequences this gene presented on chloroplast and the second region was Internal Transcribed Spacers (ITS) which present in nucleus.
The three genera under this study are two cultivars of genus Ocimum L. (Basil), two cultivars of genus Menthe L. (Mint), and two cultivars of genus Thymus L. (Thyme).
The result of the first region (rbcl) was that all samples successfully amplified the ± 630 bp fragment. Additionally, the results of alignment analysis using BLASTN tools detected that the sequence of DNA rbcL for the two local basil cultivars (Basil 1 and Basil 2) has similarities with Ocimum basilicum, Ocimum tenuiflorum, Ocimum kilimandscharicum and Ocimum gratissimum with identity 100%, 99.69%, 99.37% and 99.06%, respectively. In addition, the two local mint cultivars (Mint 1 and Mint 2) had similarities with Mentha spicata, Mentha_pulegium, Mentha canadensis and Mentha menthaefolia with identity 99.85%, 99.84%, 99.69% and 99.53%, respectively.
For thyme local cultivars (Thyme1 and Thyme2), Thyme1 cultivar sample genotype is genetic closely with species, Thymus alsinoides and Thymus sibthorpii with identity 99.69% and 99.84%, respectively and they located nearest from the cluster (Thymus genus) members in
In our study, six local Egyptian commercial cultivars of family lamiaceae were studied, this family is one of the three largest family that contain Herbal medicines (Asteraceae, Rosaceae and Lamiaceae) that have used from ancient times. Two regions sequences were analyzed to compare between species, the first region was ribulose 1,5-bisphosphate carboxylase Large (rbcL) gene at the level of DNA sequences this gene presented on chloroplast and the second region was Internal Transcribed Spacers (ITS) which present in nucleus.
The three genera under this study are two cultivars of genus Ocimum L. (Basil), two cultivars of genus Menthe L. (Mint), and two cultivars of genus Thymus L. (Thyme).
The result of the first region (rbcl) was that all samples successfully amplified the ± 630 bp fragment. Additionally, the results of alignment analysis using BLASTN tools detected that the sequence of DNA rbcL for the two local basil cultivars (Basil 1 and Basil 2) has similarities with Ocimum basilicum, Ocimum tenuiflorum, Ocimum kilimandscharicum and Ocimum gratissimum with identity 100%, 99.69%, 99.37% and 99.06%, respectively. In addition, the two local mint cultivars (Mint 1 and Mint 2) had similarities with Mentha spicata, Mentha_pulegium, Mentha canadensis and Mentha menthaefolia with identity 99.85%, 99.84%, 99.69% and 99.53%, respectively.
For thyme local cultivars (Thyme1 and Thyme2), Thyme1 cultivar sample genotype is genetic closely with species, Thymus alsinoides and Thymus sibthorpii with identity 99.69% and 99.84%, respectively and they located nearest from the cluster (Thymus genus) members in
Other data
| Title | COMPARATIVE GENOMIC STUDIES OF SOME LAMIACEAE FAMILY PLANTS | Other Titles | دراسات جينومية مقارنه على بعض أنواع نباتات العائلة الشفوية | Authors | HADER MOHAMED AHMED AHMED MANSOUR | Issue Date | 2020 |
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