Chitosan and thidiazuron improve regeneration efficiency of strawberry (Fragaria x ananassa Duch.) cv. Festival from different explant types
Youssef, Sabry Mousa Soliman;
Abstract
Establishment of an efficient regeneration system is considered as a prerequisite step for genetic
transformation and somaclonal variation which have provided alternative and efficient strategies to
the breeders for obtaining genetic variability and improving new genotypes. To optimize the
regeneration protocol for strawberry cv. Festival, different explant types, i.e. leaf discs, petioles,
stipules, and roots were cultured on N30K mineral formulation with MS microelements medium
containing different concentrations of indole-3-butyric acid (IBA) (0.2 or 0.5 mg l–1
) with thidiazuron
(TDZ) (1.0, 1.5 or 2.0 mg l–1
) or 6-benzylaminopurine (BA) (1.0, 2.0 or 3.0 mg l–1
) and supplemented
with different concentrations of chitosan (0, 25 or 50 mg l–1
). In addition, the effect of chitosan on
shoot multiplication was tested. Results clearly revealed that leaf discs proved to be a good source of
explant material for regeneration of Festival cultivar, since the regeneration efficiencies varied from
58 to 100%, from 0 to 40%, from 8.33 to 47.66%, and from 11 to 57.66%, for leaf discs, petioles,
stipules, and roots, respectively. In addition, TDZ was more effective than BA and chitosan
application acted as a growth stimulator for shoot regeneration from all tested explants. A medium
containing 0.5 mg l–1 IBA and 1.5 mg l–1 TDZ supplemented with 25 mg l–1 chitosan was found to be
the most efficient combination for the regeneration from leaf discs, petioles and stipules, and
produced the highest number of shoots/leaf disc. The medium containing 0.5 mg l–1 IBA and 2.0 mg l–
1 TDZ supplemented with 50 mg l–1 chitosan produced the highest number of shoots regenerated from
petioles and stipules, while 0.5 mg l–1 IBA and 2.0 mg l–1 TDZ combination with 25 mg l–1 chitosan
gave the highest regeneration frequency and number of shoots for roots. Moreover, chitosan
application at 25 or 50 mg l–1 had positive effect on shoot multiplication. Both concentrations
accelerated the appearance of the proliferation rate by 5-7 days and increased number of
plantlets/explant without significant differences. In conclusion, an efficient regeneration protocol was
optimized for strawberry cv. Festival from different explant types.
transformation and somaclonal variation which have provided alternative and efficient strategies to
the breeders for obtaining genetic variability and improving new genotypes. To optimize the
regeneration protocol for strawberry cv. Festival, different explant types, i.e. leaf discs, petioles,
stipules, and roots were cultured on N30K mineral formulation with MS microelements medium
containing different concentrations of indole-3-butyric acid (IBA) (0.2 or 0.5 mg l–1
) with thidiazuron
(TDZ) (1.0, 1.5 or 2.0 mg l–1
) or 6-benzylaminopurine (BA) (1.0, 2.0 or 3.0 mg l–1
) and supplemented
with different concentrations of chitosan (0, 25 or 50 mg l–1
). In addition, the effect of chitosan on
shoot multiplication was tested. Results clearly revealed that leaf discs proved to be a good source of
explant material for regeneration of Festival cultivar, since the regeneration efficiencies varied from
58 to 100%, from 0 to 40%, from 8.33 to 47.66%, and from 11 to 57.66%, for leaf discs, petioles,
stipules, and roots, respectively. In addition, TDZ was more effective than BA and chitosan
application acted as a growth stimulator for shoot regeneration from all tested explants. A medium
containing 0.5 mg l–1 IBA and 1.5 mg l–1 TDZ supplemented with 25 mg l–1 chitosan was found to be
the most efficient combination for the regeneration from leaf discs, petioles and stipules, and
produced the highest number of shoots/leaf disc. The medium containing 0.5 mg l–1 IBA and 2.0 mg l–
1 TDZ supplemented with 50 mg l–1 chitosan produced the highest number of shoots regenerated from
petioles and stipules, while 0.5 mg l–1 IBA and 2.0 mg l–1 TDZ combination with 25 mg l–1 chitosan
gave the highest regeneration frequency and number of shoots for roots. Moreover, chitosan
application at 25 or 50 mg l–1 had positive effect on shoot multiplication. Both concentrations
accelerated the appearance of the proliferation rate by 5-7 days and increased number of
plantlets/explant without significant differences. In conclusion, an efficient regeneration protocol was
optimized for strawberry cv. Festival from different explant types.
Other data
| Title | Chitosan and thidiazuron improve regeneration efficiency of strawberry (Fragaria x ananassa Duch.) cv. Festival from different explant types | Authors | Youssef, Sabry Mousa Soliman | Keywords | In vitro, Organogenesis, Adventitious shoot regeneration, Thidiazuron | Issue Date | 2016 | Journal | Middle East Journal of Agriculture Research, 5 (4): 856-867 | ISSN | 2077-4605 |
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