Evaluation of Some Biochemical Activities of Viburnum tinus leaves Extract in Vitro

Abstract

The Viburnum tinus (V.tinus) is an Adoxaceae family medicinal plant rich in vital phytochemicals such as polyphenols, triterpenoids, flavonoids, coumarins, and iridoids. This study shows that V. tinus leaf extracts have anti-inflammatory, anti-cancer, and apoptotic properties. The plant leaves were dried and soaked in 90% MeOH to prepare the crude extract, which was fractionated by n-Hexane, CH2Cl2, and MeOH. The oleanolic acid and chlorogenic acid were isolated from chloroform extract. The phytochemicals were detected in these extracts, and the anti-inflammatory, anti-cancer, and apoptotic activities were evaluated of these extracts and isolated compounds in-vitro. The highest content of flavonoids, tannins, terpenoids, and coumarins in the n-Hexane extract stimulates its anti-inflammatory activity, as evidenced by protein denaturation inhibition (94.60 ± 3.90 percent), proteinase inhibition (88.5 ± 0.50 %), membrane stabilization (73.2 ± 1.58 %), and inflammatory cytokine (INF- ɤ) inhibition (100 ± 0.00 %) without any toxicity on blood. It also has a cytotoxic impact on HepG2, MCF7, and CaCO2 cell lines, with IC50 (29.7 ± 2.58, 75.3 ± 4.70, 29.1