DETECTING OF RESISTANCE IN CERTAIN FIELD STRAINS OF PINK BOLLWORM TO SOME INSECTICIDES AND THE ROLES OF BIOCHEMICAL AND MOLECULAR FACTORS

Abstract

The present work was undertaken to study certain aspects which might affect the phenomenon of resistance or tolerance of the pink bollworm (Pectinophora gossypiella Sannd.) field populations collected from some Governorates in Egypt to certain conventional insecticides, comparing with ore and nanoparticles formulation of natural compounds. Moreover, chitosan biopolymers and it’s nanoparticles was also evaluated. The discriminating dose was using in monitoring the resistance of field population to tested insecticides and compounds. Comparative investigation of the rate of development of selected resistance strains of pink bollworm to each of organophosphorus and Pyrethroids compounds was carried out. The enzymatic activity which have a role in resistance to insecticide was investigated in certain field population. The study included also the identification and detection of genetic diversity of pink bollworm field population. Also the insecticide-resistance gene expression using Real-Time (PCR) was evaluated in certain population. The following results were obtained: