The Effect of Green Coffee Aqueous Extract on Parotid Salivary Gland of Streptozotocin Induced Diabetic Albino Rats (Histological and Immunohistochemical study)
Yasmin Mohamed Hasan Elghazawy;
Abstract
Background: Diabetes mellitus (DM) is a metabolic disorderwhose complications effect widely on the quality of life, longevity and healthcare costs. The potential of various anti-diabetic herbal which are tested for its antidiabetic efficacy have been also extensively illustrated. Coffee is one of the most consumed beverages worldwide and is one of the main sources of chlorogenic acid (CGA) especially in its green form with various biological benefits so it’s expected to reduce the damaging effect of DM in parotid salivary gland.
Objectives: The aim of the present study was to evaluate the possible effect of green coffee beans aqueous extract on parotid salivary gland of streptozotocin induced diabetic albino rats.
Material and methods:Forty two adult male albino rats were divided equally into 3 main groups (14 rats each). Group I (control) rats received 0.2-0.25ml citrate buffer. This group was divided equally into 2 subgroups IA, IB in which rats were sacrificed corresponding to their experimental subgroups. Group II (diabetic) rats injected by single dose of STZ (40mg/kg). Group III(diabetic treated) rats received green coffee at a dose of 93mg/kg in form of aqueous extract 1.24-1.55mlorally once a day from the development of diabetes till the day of sacrifice. In group II and III rats were divided into subgroup A and B (rats sacrificed after 1 and 2 weeks of development of diabetes, respectively). Parotid glands were dissected and examined histologically and immunohistochemically.
Results:Histologically, serous acini of group II showed histological deterioration in form of pleomorphic nuclei and cytoplasmic vacuolations.In group III,serous acini showed less nuclear changes and minimal vacuolations.Immunohistochemically,PGs of group II showed positive diffuse cytoplasmic reactions ofcaspase-3 in theirparenchymal elements, while the nuclear reactions were localized in group III. Statistically, area% of both anti-active caspase-3 antibody and inter-acinar spaces showed significant increase in subgroup IIB and significant decrease in subgroup IIIB.
Conclusions: Green coffee aqueous extract can decrease the deteriorative effects of diabetes on parotid salivary glands in time dependent manner
Objectives: The aim of the present study was to evaluate the possible effect of green coffee beans aqueous extract on parotid salivary gland of streptozotocin induced diabetic albino rats.
Material and methods:Forty two adult male albino rats were divided equally into 3 main groups (14 rats each). Group I (control) rats received 0.2-0.25ml citrate buffer. This group was divided equally into 2 subgroups IA, IB in which rats were sacrificed corresponding to their experimental subgroups. Group II (diabetic) rats injected by single dose of STZ (40mg/kg). Group III(diabetic treated) rats received green coffee at a dose of 93mg/kg in form of aqueous extract 1.24-1.55mlorally once a day from the development of diabetes till the day of sacrifice. In group II and III rats were divided into subgroup A and B (rats sacrificed after 1 and 2 weeks of development of diabetes, respectively). Parotid glands were dissected and examined histologically and immunohistochemically.
Results:Histologically, serous acini of group II showed histological deterioration in form of pleomorphic nuclei and cytoplasmic vacuolations.In group III,serous acini showed less nuclear changes and minimal vacuolations.Immunohistochemically,PGs of group II showed positive diffuse cytoplasmic reactions ofcaspase-3 in theirparenchymal elements, while the nuclear reactions were localized in group III. Statistically, area% of both anti-active caspase-3 antibody and inter-acinar spaces showed significant increase in subgroup IIB and significant decrease in subgroup IIIB.
Conclusions: Green coffee aqueous extract can decrease the deteriorative effects of diabetes on parotid salivary glands in time dependent manner
Other data
| Title | The Effect of Green Coffee Aqueous Extract on Parotid Salivary Gland of Streptozotocin Induced Diabetic Albino Rats (Histological and Immunohistochemical study) | Other Titles | تأثير المستخلص المائي للبن الأخضر على الغده اللعابيه النكافيه في الفئران البيضاءالمصابه بالسكري المستحث بالستريبتوزوتوسين. (دراسة هستولوجية وهيستوكيميائية مناعية) | Authors | Yasmin Mohamed Hasan Elghazawy | Issue Date | 2020 |
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